Caribou Biosciences, Inc.

Columbia University

Sanne Eveline Klompe

Samuel Henry Sternberg

Peter Sean Cameron

Scott David Gradia

Matthew Scott Thompson

Samuel Henry Stemberg

In certain embodiments, the present systems and methods use Tn7-like transposons that encode CRISPR-Cas systems for programmable, RNA-guided DNA integration. For example, the CRISPR-Cas machinery directs the Tn7 transposon-associated proteins to integrate DNA downstream of a target site (e.g., a genomic target site) recognized by a guide RNA (gRNA).

RNA-guided DNA integration using Tn7-like transposons

The present disclosure provides engineered Class 1 Type I CRISPR-Cas (Cascade) systems that comprise multi-protein effector complexes, nucleoprotein complexes comprising Type I CRISPR-Cas subunit proteins and nucleic acid guides, polynucleotides encoding Type I CRISPR-Cas subunit proteins, and guide polynucleotides. Also, disclosed are methods for making and using the engineered Class 1 Type I CRISPR-Cas systems of the present invention.

Engineered cascade components and cascade complexes

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New York, NY, United States of America

Sanne Eveline Klompe