City of Hope

Sanjeet Dadwal

Markus Kalkum

Karine Bagramyan

James I Ito

Diana Diaz-Arevalo

Miro Rusnak

Isaac Bishara

Daniel Roeth

Provided are methods, devices, and systems for a loop-mediated isothermal amplification (LAMP) reactions for detecting the presence of genetic material. A LAMP device includes an enclosure having outer walls configured to define an internal space. The LAMP device also includes a tray assembly configured to support a reaction vessel configured for holding a sample for amplification. The LAMP device also includes a plurality of light emitters configured to illuminate the reaction vessel for exciting a fluorescence of the sample in the reaction vessel. The LAMP device also includes an imaging device configured to obtain an image of the sample for determining a quantity of the fluorescence in the sample. The LAMP device also includes a controller configured to regulate a temperature of the tray assembly and instruct the imaging device to obtain the image for determining the quantity of the fluorescence in the sample.

Loop-mediated isothermal amplification devices and systems for detecting genetic material

Provided herein are methods for detecting anprotease in a sample, diagnosing a subject with aspergillosis caused by aninfection based on the presence of anprotease in a sample, and methods of aspergillosis treatment that incorporate these diagnostic methods. In certain embodiments, theprotease is Asp f2, and theinfection is caused, or. Also provided herein are antibodies and kits for use in these methods, including novel antibodies specific for Asp f2.

Methods, compositions, and kits for detection of aspergillosis

Growing community of inventors

Duarte, CA, United States of America

Sanjeet Dadwal