The Public Health Research Institute of the City of New York, Inc.

Rutgers, the State University of New Jersey

The Salk Institute for Biological Studies

Phri Properties, Inc.

Other

City University of New York

The Public Health Research Institute

The Public Health Research Institute of New York, Inc.

Sanjay Tyagi

Fred Russell Kramer

Paul M Lizardi

Salvatore A Marras

Diana Vargas-Gold

David Alland

Salvatore A E Marras

Maria Laura Gennaro

Cesar E Guerra

Robert Vartikian

Hilda M Buyoli

Ulf D Landegren

Yuri Bushkin

Richard Pine

Jack W Szostak

Gerald F Joyce

Leslie E Orgel

Barbara C Chu

Osama A Alsmadi

Arjun Raj

Hiyam Elhajj Trunfio

Herman J Blok

Jacqueline Vet

Amy Piatek

Salvatore AE Marras

The present invention relates to a method for rapidly detecting copies of at least one RNA molecule expressed in individual cells and uses thereof.

Rapid assays for T-cell activation by RNA measurements using flow cytometry

This invention discloses multi-part primers for primer-dependent nucleic acid amplification methods. Also disclosed are multiplex assay methods, related reagent kits, and oligonucleotides for such methods.

Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor

The present invention relates to the detection of nucleic acids sequences in situ using hybridization probes and generation of amplified hybridization signals, wherein background signal is reduced and sensitivity is increased.

Target mediated in situ signal amplification with dual interacting hairpin probes

This invention discloses multi-part primers for primer-dependent nucleic acid amplification methods. Also disclosed are primer-dependent nucleic acid amplification reactions, particularly DNA amplification reactions, reaction mixtures and reagent kits for such reactions. This invention relates to primer-dependent nucleic acid amplification reactions, particularly DNA amplification relations such as PCR, and primers, reaction mixtures and reagent kits for such reactions and assays employing same.

Highly selective nucleic acid amplification primers

This invention discloses multi-part primers for primer-dependent nucleic amplification methods. Also disclosed are primer-dependent nucleic acid amplification reactions, particularly DNA amplification reactions, reaction mixtures and reagent kits for such reactions. This invention relates to primer-dependent nucleic acid amplification reactions, particularly DNA amplification reactions such as PCR, and primers, reaction mixtures and reagent kits for such reactions and assays employing same.

This invention discloses multi-part primers for primer-dependent nucleic acid amplification methods. Also disclosed are primer-dependent nucleic acid amplification reactions, particularly DNA amplification reactions, reaction mixtures and reagent kits for such reactions. This invention relates to primer-dependent nucleic acid amplification reactions, particularly DNA amplification reactions such as PCR, and primers, reaction mixtures and reagent kits for such reactions and assays employing same.

A method for probing a target sequence of messenger ribonucleic acid molecules (mRNA's) in a fixed, permeabilized cell, said target sequence including at least 30 non-overlapping probe binding regions of 15-100 nucleotides, comprising immersing said cell in an excess of at least 30 nucleic acid hybridization probes, each singly labeled with the same fluorescent label and each containing a nucleic acid sequence that is complementary to a different probe binding region of said target sequence; washing said fixed cell to remove unbound probes; and detecting fluorescence from said probes.

Imaging individual mRNA molecules using multiple singly labeled probes

The invention provides assays that can detect multiple genetic variants of a gene (e.g., a mycobacterium gene) in a sample using a pool (using 2, 3, 4, or more) of oligonucletide hybridization probes.

Assays for short sequence variants

A coding scheme for microcarriers suitable for use in distributed arrays includes labeling the carriers with quenched signaling hairpin molecules with any one of three to eight distinguishable fluorophores wherein the hairpins are of at least two types, most preferably three types, that open and fluoresce differentially as a chemical or physical condition, for example temperature, is changed. Mixtures of microcarriers having immobilized capture probes can be decoded by measuring fluorescence from said fluorophores under conditions under which only one type of hairpin is open, under which two types of hairpin are open, and so on. Mixtures of coded microcarriers with capture probes are used in assays for nucleic acids utilizing microarray methods.

Optically decodable microcarries, arrays and methods

The invention provides assays that can detect multiple genetic variants of a gene (e.g., a mycobacterial gene) in a sample using a pool (e.g., 2, 3, 4, or more) of oligonucleotide hybridization probes.

Coalescence of cells or other membrane-bound entities is facilitated by anchoring an outwardly projecting first oligonucleotide in one member and an outwardly projecting second oligonucleotide, complementary to the first, in a second member and incubating under hybridizing conditions. Liposomes may be coalesced with cells to deliver hydrophilic agents thereto, such as DNA probes or drugs. Kits containing complementary oligonucleotides containing hydrophobic anchoring moieties may be used.

Oligonucleotide-facilitated coalescence

Non-competitive, quantitative amplification assay methods, including assays employing amplification by the polymerase chain reaction (PCR) process, for accurately measuring levels of target nucleic acid and sequences in samples and for ascertaining the relative amounts of cross-hybridizing alleles and mutants.

Non-competitive co-amplification methods

For nucleic acid amplification including extension of primers by a DNA polymerase, high specificity primers are provided. The primers include a type of hairpin structure in which a single-stranded loop separates complementary 3&prime; and 5&prime; arms and in which the loop and the 3&prime; arm are complementary to the target nucleic acid. Amplification methods, assays and kits including such primers are included in the invention.

Growing community of inventors

New York, NY, United States of America

Sanjay Tyagi