Molecular Assemblies, Inc.

Sanjay Agarwalla

J William Efcavitch

Kim F Albizati

Natasha Paul

Matthew T Holden

Alan Grubbs

Patrycja A Hopkins

Jay K Singh

The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using renewable initiators coupled to a solid support. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template.

Reusable initiators for synthesizing nucleic acids

Nucleic acid memory strands encoding digital data using a sequence of homopolymer tracts of repeated nucleotides provides a cheaper and faster alternative to conventional digital DNA storage techniques. The use of homopolymer tracts allows for lower fidelity, high throughput sequencing techniques such as nanopore sequencing to read data encoded in the memory strands. Specialized synthesis techniques allow for synthesis of long memory strands capable of encoding large volumes of data despite the reduced data density afforded by homopolymer tracts as compared to conventional single nucleotide sequences.

Homopolymer encoded nucleic acid memory

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San Diego, CA, United States of America

Sanjay Agarwalla