Takara Bio Inc.

Rutgers, the State University of New Jersey

University of Medicine and Dentistry of New Jersey

Sangita Phadtare

Masayori Inouye

Ikunoshin Kato

Takashi Uemori

Hiroyuki Mukai

Kazue Nishiwaki

Kunitoshi Yamanaka

Ling Zhu

Bing Xia

Guoliang Qing

Haiping Ke

The present invention provides cold shock protein-containing compositions for improved DNA synthesis reactions with improved reactivity, methods for synthesizing DNA using such compositions, kits for use in such methods, and DNA compositions yielded by such methods. The present invention further provides cold shock protein-containing compositions for the identification of endoribonuclease cleavage sites, methods for identifying endoribonuclease cleavage sites using such compositions, and kits for use in such methods.

Cold shock protein compositions and methods and kits for the use thereof

The present invention relates to a DNA molecule or vector and a host cell containing this DNA molecule or vector which can be used to produce a heterologous polypeptide under conditions that elicit a cold shock response in the host cell. The DNA molecule and vector include a nucleotide sequence encoding a heterologous polypeptide and a promoter and 5'-UTR from a cold shock inducible gene which directs its expression. In addition, an AT-rich sequence that enhances translation under cold shock inducible conditions is either present in the coding sequence of the heterologous polypeptide or in an additional element inserted between the coding sequence and the cold shock inducible promoter and 5&#x2032;-UTR.

Cold shock inducible expression and production of heterologous polypeptides

The invention relates to a gene isolated from, dep, which confers resistance to the antibacterial activity of 4,5-dihydroxy-2-cyclopenten-1-one (DHCP). The invention further relates to the putative protein encoded by dep, which is a hydrophobic, transmembrane efflux protein specific for DHCP. The invention further relates to plasmids containing the dep gene, and to bacterial cells expressing dep. Furthermore, the invention provides applications for use in conferring resistance to antibacterial activity in organisms. The dep gene can be used to identify compounds which inhibit the efflux activity responsible for the resistance to DHCP or to compounds which are functionally equivalent to DHCP.

Method of using a gene conferring resistance to 4,5-dihydroxy-2-cyclopenten-1-one (DHCP) and identifying inhibitors of a polypeptide encoded by the gene

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Highland Park, NJ, United States of America

Sangita Phadtare