Lg Chem, Ltd

Korea Advanced Institute of Science and Technology

Medigenes

Sang-Yup Lee

Si-Jae Park

Sang-Hyun Lee

Taek-Ho Yang

Tae-Wan Kim

Eun-Jeong Lee

Hye-Ok Kang

Ki-Jun Jeong

Tae Yong Kim

Dong-Yup Lee

Ho-Nam Chang

Won-min Yoo

Yu-Kyung Jung

Nagendra Narayan Thakur

Jin-Hwan Do

Jun-Hyeong Cho

Ki-Chang Keum

Nae-Choon Yoo

Hyun-Cheol Chung

The present invention relates to cells or plants that can produce polylactate or its copolymers and to a method for preparing polylactate or its copolymers using the same. More specifically, cells or plants with the ability to produce polylactate or hydroxyalkanoate-lactate copolymers comprise both a gene encoding an enzyme that converts lactate into lactyl-CoA and a gene encoding polyhydroxyalkanoate (PHA) synthase which uses lactyl-CoA as a substrate. Also described is a method for preparing polylactate or hydroxyalkanoate-lactate copolymers which comprises culturing the cells in a medium containing lactate or lactate and various hydroxyalkanoates or culturing the plants. Effective preparation of hydroxyalkanoate-lactate copolymer which comprises various hydroxyalkanoates as well as polylactate, using the cells or the plants, is disclosed.

Cells or plants producing polylactate or its copolymers and uses thereof

A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.

Mutants having capability to produce 1, 4-butanediol and method for preparing 1, 4-butanediol using the same

The present invention relates to a copolymer comprising 3-hydroxyalkanoate monomer unit and lactate monomer unit, or their preparing method. More specifically, the present invention relates to a method for preparing a copolymer comprising lactate monomer and 3-hydroxyalkanoate monomer, wherein the method comprises culturing a cell or plant comprising the gene of enzyme converting lactate and 3-hydroxyalkanoate into lactyl-CoA and 3-hydroxyalkanoyl-CoA, respectively, and polyhydroxyalkanoate synthase gene together, and the copolymer made by the method. The copolymer of the present invention is a biodegradable polymer being able to be usefully used instead of conventional synthetic plastic, and the copolymer can be used also for medical use.

Copolymer containing 3-hydroxyalkanoate unit and lactate unit, and its manufacturing method

The present invention relates to a method for improving an organism through the profiling of flux sum of metabolites, and more particularly to a method for screening key metabolites, the method comprises: plotting a profile between objective functions based on useful substance formation rate as a main function through an algorithm perturbing other functions influencing the production of useful substance; determining the utilization (flux sum (&#x3a6;)) of all metabolites from the profile; and screening key metabolites, which show an increase in flux sum (&#x3a6;) with an increase in useful substance formation rate. The present invention also relates to a method for improving an organism producing a useful substance, the method comprises introducing and/or amplifying genes associated with the screened key metabolites or introducing the genes from the outside into the organism. According to the disclosed invention, the metabolic utilization (flux sum; &#x3a6;) of specific metabolites according to an increase in useful substance formation rate can be predicted, so that key metabolites in increasing the production of a useful substance can be screened. Also, it is possible to increase the production of a useful substance through the method of improving a target organism by introducing and/or amplifying genes associated with the screened metabolites or through the method of supplying the metabolites during the culture of the organism.

Method of improvement of organisms using profiling the flux sum of metabolites

The present invention relates to polyhydroxyalkanoate synthase (PHA synthase) mutant originated fromsp. 6-19 (KCTC 11027BP) which can prepare lactate polymer and/or copolymer by using lactyl-CoA as a substrate. The present invention relates to a method for preparing lactate polymer and/or copolymer with the synthase mutant. The polyhydroxyalkanoate synthase mutants of the present invention originated fromsp. 6-19 can efficiently prepare lactate polymer and/or copolymer by using as a substrate lactyl-CoA which is difficult to be used as a substrate by conventional polyhydroxyalkanoate synthase.

[object Object]

Provided are a microorganism capable of producing a polylactate (PLA) or PLA copolymer from sucrose and a method of producing the PLA or PLA copolymer from sucrose using the same. A gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate are introduced into a microorganism capable of using sucrose as a substrate, and the microorganism is cultured using sucrose as the substrate, thereby allowing efficient production of a PLA or PLA copolymer.

Recombinant microorganism capable of producing polylactate or polylactate copolymer from sucrose and method for producing polylactate or polylactate  copolymer from sucrose using the same

The present invention relates to a copolymer comprising 4-hydroxybutyrate monomer unit and lactate monomer unit, a copolymer 4-hydroxybutyrate monomer unit, lactate monomer unit and 3-hydroxyalkanoate, or their preparing method. More specifically, the present invention relates to a method for preparing a copolymer comprising lactate monomer; 4-hydroxybutyrate monomer; and optionally 3-hydroxyalkanoate, wherein the method comprises culturing a cell or plant comprising the gene of enzyme converting lactate and 3-hydroxyalkanoate into lactyl-CoA and 3-hydroxyalkanoyl-CoA, respectively, phosphotransbutylase gene, butyrate kinase gene and polyhydroxyalkanoate synthase gene together, and the copolymer made by the method. The copolymer of the present invention is a biodegradable polymer being able to be usefully used instead of conventional synthetic plastic, and the copolymer can be used for medical use.

Copolymer comprising 4-hydroxybutyrate unit and lactate unit and its manufacturing method

The present invention provides an expression vector comprising genes encoding OmpF ofand a desired protein,transformed with the expression vector, and a method for extracellular production of desired proteins by employing the same. The recombinant expression vector of the invention comprises an ampicillin-resistance gene, the OmpF promoter and the OmpF gene. In accordance with the invention, a desired protein can be produced extracellularly by a simpler method than conventional methods such that: secretory production of OmpF fusion protein begins simultaneously with growth of the cells through constitutive expression employing an OmpF promoter, and as the concentration of cells increases, the amount of secretory production of the protein also increases continuously. Therefore, desired proteins can be produced in large quantities by a high concentration culture of cells.

The present invention provides a recombinant plasmid vector comprising a kanamycin resistance gene, a promoter, an endoxylanase signal sequence, a nucleotide sequence coding for an oligopeptide consisting of 13 amino acids including 6 consecutive histidine residues, and a human granulocyte colony stimulating factor(hG-CSF) gene; antransformed with the said vector; and, a process for producing complete hG-CSF protein with high purity from the protein pool secreted by the said microorganism. In accordance with the invention, the hG-CSF protein can be prepared with high purity through rather simple process facilitating secretion of large amount of hG-CSF fusion protein into the periplasm, which does not require complicated processes such as solubilization and subsequent refolding required for isolation of the hG-CSF protein produced in cytoplasm as insoluble inclusion bodies by conventional techniques, thus, the hG-CSF protein can be widely used as an active ingredient in the development of supplementary agents for anticancer therapy.

The present invention provides a method for manufacturing highly-concentrated polyglutamic acid by culturing an aerobic micro-organism ofsp. with the additional supply of saccharides. The method for manufacturing polyglutamic acid comprises a step of culturingsp. in a fed-batch or batch culture while supplying saccharides to the culture. Since the method for manufacturing highly-concentrated polyglutamic acid can be applicable to the industrial scale fermentation, mass production of polyglutamic acid can be feasible in a cost-efficient way.

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Daejeon, South Korea

Sang-Yup Lee