Chonnam National University

Sang-Oh Ahn

Geun Joong Kim

Dae Eun Cheong

Sung Hwan You

Chul-Ho Yun

Su-Kyoung Yoo

Hun-Dong Lee

Hye-Ji Choi

Ho-Dong Lim

A method of expressing hydrophobin in a soluble form and a method of purifying hydrophobin, and more particularly, to a method of expressing a recombinant fusion protein including hydrophobin in a soluble form in a host cell and then purifying the expressed recombinant fusion protein are provided.

Method for soluble expression and purification of hydrophobin

An NADPH-regeneration system based on monomeric isocitrate dehydrogenase (IDH) and a use thereof. Specifically, the present invention relates to a recombinant vector including a polynucleotide encoding an isocitrate dehydrogenase recombinant protein derived from(CgIDH) and an isocitrate dehydrogenase recombinant protein derived from(AvIDH), a method for producing the recombinant protein, and an NADPH-regeneration system using the recombinant protein produced by the method. The enzyme in a monomeric form that may be efficiently used in the NADPH-regeneration system in the transformant into which the recombinant vector was introduced, was found, and the NADPH-regeneration system using the enzyme in a monomeric form has a very high utility value as biological parts and biocatalyst materials that provides NADPH to the NADPH-dependent enzyme.

Methods of use for an NADPH-regeneration system based on a monomeric isocitrate dehydrogenase

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Gwangju, South Korea

Sang-Oh Ahn