Chong Kun Dang Pharmaceutical Corporation

Engene, Inc.

Sang Myoung Noh

Young Nam Lee

Min Hyo Ki

Ho Young Hwang

Jong Lae Lim

Yu-Kyoung Oh

Myung Suk Kim

Ji Hee Lee

Myung-Ok Park

So Hyun Park

Hee-Jeong Cho

Ji Hye Hwang

Ki Seong Ko

Ah-Reum Chae

Sung Sik Bang

Tae Hyun Park

Ji Hyeh Hwang

Disclosed herein is a composition for gene delivery which forms into liquid crystals in an aqueous fluid including chitosan and a liquid crystal formation material. The composition of the present invention including chitosan and a liquid crystal formation material improves the relatively low binding strength between chitosan and a gene, and also considerably increases the stability of a chitosan nanocomposite in blood serum, thereby performing highly efficient gene delivery and providing excellent stability, thus being useful as a gene therapeutic agent.

Composition for gene delivery comprising chitosan and liquid crystal formation material

Disclosed is a pharmaceutical composition for gene delivery, comprising: (i) a gene; (ii) a water-soluble chitosan; (iii) a thiamine pyrophosphate or a pharmaceutically acceptable salt thereof; (iv) a protamine or a pharmaceutically acceptable salt thereof; and (v) a neutral or anionic phospholipid. The composition can introduce a gene into cells safely and effectively. Composed of non-toxic and injectable components, the composition is safe for the body and can be advantageously commercialized. Notably, it can deliver a gene at high efficiency in vivo as well as in vitro, and is stable in the blood.

Composition for gene delivery

The present invention relates to a conjugate of chitosan and polyamine polymer that is useful for transferring a desired gene medicine into cells, and a method for preparing the same. In particular, the present invention relates to a double conjugate that is prepared by 1 in king poly-L-arginine to low molecular weight chitosan or triple conjugate that is prepared by additionally linking polyethylene glycol (PEG) to the double conjugate, and a method for preparing the same. The chitosan based cationic polymer conjugate of the present invention forms a complex with negatively charged gene medicine such as plasmid DNA and small interfering RNA to efficiently transfer the desired gene medicine into cells with low cytotoxicity. Accordingly, the conjugate can be used as an effective delivery system for in vivo administration of gene medicine.

Chitosan based polymer conjugate and a method for producing the same

Growing community of inventors

Seoul, South Korea

Sang Myoung Noh