Danisco US Inc.

The Procter Gamble Company

Wildes, David E.

Cuevas, William

Lee, Sang-kyu

Sharma, Vivek

Finan, Dina

Sang-Kyu Lee

David Aaron Estell

Michael C Ward

Michael D Ward

Louise Wallace

Andrew Shaw

Eugene Steven Sadlowski

Hiroshi Oh

William Albert Cuevas

Sandra W Ramer

Amr R Toppozada

Scott Douglas Power

Luis Gustavo Cascao-Pereira

Ronaldus W J Hommes

Ronaldus Wilhelmus Joannes Hommes

Michael Jay Pepsin

Carol A Requadt

Regina Chin

David E Estell

Vivek Sharma

Mark S Gebert

David E Wildes

Mariliz Johnson

Dina Finan

Sura H Hadi

Mariliz Ortiz-Maldonado

Ronald Hommes

William A Cuevas

William Cuevas

Sura Hussain Hadi

The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained fromsp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from. In still further preferred embodiments, the neutral metalloprotease is a variant of theneutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the theneutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

Use and production of storage-stable neutral metalloprotease

Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.

Alpha-amylase combinatorial variants

The present teachings provide modified enzymes, preferably phytases, which have increased stability, hypothesized to arise from increased glycosylation. The enzymes can be modified to introduce or increase the number of glycosylation sites in the amino acid sequence, or glycosylation can be increased by the use of specific host production methods, or both. The enzymes of the present teachings have an increased stability after treatment at elevated temperature, which can be measured by inactivity reversibility or percent recovery following a treatment such as heating. The enzymes of the present teachings find application for example in food, feed, and feed pelleting.

Glycosylation as a stabilizer for phytase

Alpha-amylases from(AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.

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An &#x3b1;-amylase from(AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.

The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained fromsp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from. In still further preferred embodiments, the neutral metalloprotease is a variant of theneutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of theneutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

Thermostable neutral metalloproteases

Described are variants of a parent &#x3b1;-amylase that exhibits an alteration in at least one of the following properties relative to said parent &#x3b1;-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, Ca2+ dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.

Growing community of inventors

Palo Alto, CA, United States of America

Sang-Kyu Lee