Korea Research Institute of Bioscience and Biotechnology

Dongkook Pharmaceutical Co., Ltd.

Other

Korea Institute of Science and Technology

Bioland Ltd.

Sang Ki Rhee

Hyun Ah Kang

Myung K Kim

Wan Soo Kim

Bong Hyun Chung

Jin Kyu Park

Hye Yun Moon

Hak Ryol Kim

Joo Hyung Heo

Eun Jung Kim

T B Panichkina

Doo Byoung Oh

Won T Cho

Moo Woong Kim

A G Domracheva

L A Saburoba

Jeong Seok Park

L M Nobikoba

Jung Hoon Sohn

Y E Bartochevichi

Eui Sung Choi

Ki Ho Kim

Chul Ho Kim

Doo-Byoung Oh

Chang Seo Park

Hyun Ah Kana

Deok Joong Youn

Hae Don Lee

Hye-Yun Moon

Soo Nam Park

Kang Ll Ko

Jung Hoon Bae

Su Min Ko

Sang Min Lee

Hyun Ah Department Of Life Science Kang

Hye Yun Department Of Life Science Moon

The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using asystem. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent &#x3b1;-1,3-mannosyltransferase gene from, constructing amutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.

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The present invention relates to a novelgene regulating Unfolded Protein Response (UPR) and a method for improving the efficiency of secretory expression of a recombinant protein using the same, more particularly, a method for improving the efficiency of secretory expression of a recombinant protein by identifying, modifying, and optimizing the HpHAC1 gene, which encodes a key transcription regulatory factor of the UPR mechanism in, and a regulatory mechanism mediated by the gene. According to the present invention, the secretory expression system ofcan be used for the mass-production of secretory proteins for industrial and medical applications, thereby producing a large amount of the proteins at low cost. Accordingly, the method is employed in the production of the proteins for industrial and medical use, so as to reduce economic burden for a patient, and contribute to the improvement in the welfare of all mankind.

The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using asystem. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent &#xa5;&#xe1;-1,3-mannosyltransferase gene from, constructing amutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.

The present invention is related to novel cosmetic composition comprising beta-fructosyl-L-ascorbic acid derivatives produced by fructose conversion reaction using by ascorbic acidskin whitening cosmetic composition as an cream, skin, lotion, pack and the like.

Cosmetic composition comprising beta-fructosyl-L-ascorbic acid for skin whitening

Novelmutant strains are provided for producing human parathyroid hormone. The novel strains acre genetically disrupted in at least one of the genes encoding the yapsin family of proteases Yapsin 1, Yapsin 2 and Yapsin 3 and harbor a human parathyroid hormone gene in their genomes. Culturing the novel strains results in the secretion of intact hPTH into culture media at high yield.

Yeast transformant producing recombinant human parathyroid hormone and method for producing the hormone

Expression cassettes for transforming and their use are disclosed. The present invention relates to expression cassettes containing ribosomal DNA fragment, CYH gene resistant to cycloheximide, and a desired gene. Expression cassette further comprising GAPDH promoter gene which allows an increase in the expression of the desired gene also provided. Moreover, the present invention provides a process for producing tetraacetyl phytosphingosine using transformed cells with the expression cassettes in a higher yield.

Plasmid for gene expression in pichia ciferrii and transformation method using the same

Disclosed herein is a process for purifying hirudin from a solution containing hirudin and other substances, characterized in that the process comprises a step of subjecting the solution to a metal ion affinity chromatography wherein copper ion (Cu.sup.++) is used as a metal ion and a phosphate buffer is used as an eluent.

Purification process for hirudin using affinity chromatography

Disclosed herein are a novel strain of Streptomyces and a method for the production of aclacinomycins A, B, Y and aglycone thereof by cultivating the same. Streptomyces lavendofoliae DKRS(KCTC 0092BP) of the present invention is capable of producing aclacinomycins A, B, Y and aglycone with higher yield. Further, it is possible to selectively produce aclacinomycin A or Y by adjusting pH of the cultured broth of Streptomyces lavendofoliae DKRS to 4.4 or 4.6 with acetate buffer or hydrochloric acid, respectively.

Method for producing aclacinomycins A, B, Y using Strepomyces

Disclosed herein are a novel strain of Streptomyces and a method for the production of aclacinomycins A, B, Y and aglycone thereof by cultivating the same. Streptomyces lavendofoliae DKRS (KCTC 0092BP) of the present invention is capable of producing aclacinomycins A, B, Y and aglycone with higher yield. Further, it is possible to selectively produce aclacinomycin A or Y by adjusting pH of the cultured broth of Streptomyces lavendofoliae DKRS to 4.4 or 4.6 with acetate buffer or hydrochloric acid, respectively.

S. lavendofoliae DKRS cell culture producing anti-tumor aclacinomycins

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Seoul, South Korea

Sang Ki Rhee