The Rockefeller University

Michigan State University

Sanford M Simon

Melvin S Schindler

Yu Lin Chen

Melvin M Schindler

Yu Chen

Genetically-modified mammals and immune cells are provided which are capable of producing or secreting detectably-labeled immunoglobulin molecules as a result of genetic modifications of at least one immunoglobulin gene in the genome thereof, such that a fusion polynucleotide encoding a detectable protein or peptide and an immunoglobulin component molecule is present.

Animals, cells and methods for production of detectably-labeled antibodies

The present invention discloses the mechanism of tamoxifen mediated inhibition of acidification. This mechanism information is then used to design methods of identifying compounds that will have the pharmaceutical effect of tamoxifen without the corresponding side-effects.

Methods of identifying modulators of the estrogen receptor

The invention discloses a fluorescent bead that can be used for determining the temperature and/or metabolic state of a single cell contained in a cell/tissue sample. The fluorescent bead contains at least one temperature-sensitive fluorophore. The invention also includes methods of monitoring cellular metabolism and methods of diagnosing abnormal metabolism of a cell.

Fluorescent bead for determining the temperature of a cell and methods

The predominant effect of pH change on multidrug resistance (MDR) independent of active drug efflux has been demonstrated in tumor cells through studies of the drugs doxorubicin and daunomycin. Specifically, the distribution of both drugs was monitored in fibroblasts and myeloma cells to examine the role of pH in drug partitioning. The invention comprises in one aspect the treatment of MDR by administering a therapeutically effective amount of a pH modulator. Diagnostic utilities are contemplated and extend to drug discovery assays and methods for measuring monitoring the status of the onset or development of pH-related conditions such as MDR, as well as the measurement of intracellular drug accumulation. Therapeutic compositions include a composition comprising a pH modulator alone or in combination with the dose-limited therapeutic agent(s), and a pharmaceutically acceptable excipient, are also contemplated.

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New York, NY, United States of America

Sanford M Simon