Centre National De La Recherche Scientifique

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Institut National Des Sciences Appliquees De Toulouse

Institut National De La Recherche Agronomique

Centre National De La Recherche Scientifique-cnrs

Institute National Des Sciences Appliquees De Toulouse

Sandrine Morel

Magali Remaud-Simeon

Pierre Monsan

Claire Moulis

Marlene Vuillemin

Denis Pompon

Isabelle Andre

Philippe Urban

Yoan Brison

Emmanuelle Cambon

Yannick Malbert

Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) an an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.

Polypeptide having the ability to form connections of glucosyl units in alpha-1,3 on an acceptor

Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) and an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.

Polypeptide having the capacity to form alpha-1,3 glucosyl unit branchings on an acceptor

The invention relates to a method for producing derivatives of O-&#x3b1;-glucosylated flavonoid, comprising at least one step of incubating a glucansucrase with a flavonoid and at least one sucrose, the flavonoid being a flavonoid which is monohydroxylated or hydroxylated in a non-vicinal manner on the B cycle. The invention also relates to novel O-&#x3b1;-glucosylated flavonoid derivatives, and to the use thereof. 

Flavonoids O-A-glucosylated on the B cycle, method for the production thereof and uses

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Auzeville-Tolosane, France

Sandrine Morel