Proteus S.a.

Sandrine Dautel

Jean-Michel Masson

Fabrice Lefevre

Daniel Dupret

Cécile Persillon

Jean François Bloch

Jean Fran&ccedil;ois Bloch

The invention concerns a method for detecting a known function, from nucleic acids present in a sample, characterized in that it comprises the following steps: (a) preparing, from the nucleic acids of the sample, nucleic acid molecules comprising the gene(s) coding for the protein(s) corresponding to said function, and the control elements required for the transcription and the translation of said gene(s); (b) in vitro transcription and translation of the nucleic acid molecule prepared in step (a); (c) detecting and/or measuring the function corresponding to the protein(s) produced in step (b).

Method for detecting and/or quantifying a known function from a nucleic acid sample

The present invention relates to a method of detection in vitro of a target substance in a sample. The sample can comprise, among other things, a nucleic sequence or more generally any type of substance. In particular, the present invention provides a method of detection comprising (i) specificly labeling a substance with a reporter gene and any sequences necessary for the in vitro expression of the reporter gene; (ii) in vitro transcription and translation of the reporter gene; and (iii) in vitro detection of a reporter protein coded by the reporter gene.

METHOD OF DETECTION IN VITRO OF A TARGET SUBSTANCE IN A SAMPLE COMPRISING THE LABELLING OF SAID SUBSTANCE WITH A REPORTER GENE AND WITH THE SEQUENCES NECESSARY FOR THE EXPRESSION OF SAID REPORTER GENE IN VITRO

The invention concerns a method for determining the activity of a substance using a functional test, characterized in that it consists in detecting and/or measuring the variation of a known function corresponding to one or several proteins produced in vitro in the presence and in the absence of said substances or to the substance in the presence or in the absence of one or several proteins produces in vitro.

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Nîmes, France

Sandrine Dautel