Danisco US Inc.

The Procter Gamble Company

Danisco US Inc., Genencor Division

Danisco US Inc (157111)

Genencor International, Inc.

Sandra W Ramer

Andrew Shaw

Scott Douglas Power

William Albert Cuevas

Sang-Kyu Lee

Amr R Toppozada

Brian E Jones

Walter Weyler

Louise Wallace

David Aaron Estell

Michael Jay Pepsin

Luis Gustavo Cascao-Pereira

Gregory Scot Miracle

Jeffrey John Scheibel

Richard Ray Bott

Ayrookaran J Poulose

Clement K Choy

Gregory Marshall Whited

Thomas P Graycar

Hiroshi Oh

Chris Leeflang

Neelam S Amin

Melodie Estabrook

Carol A Requadt

Marc Eric Gustwiller

Klaus Liebeton

Claudine Chang

Edward M Concar

Jayarama K Shetty

James T Kellis, Jr

Marc Kolkman

Marguerite A Cervin

Karsten Matthias Kragh

Vivek Sharma

Casper Vroemen

Brian James Paul

Michelle Jackson

Donald E Ward

Matthew Grant Boston

Casper Willem Vroemen

Regina Chin

Mansi Goyal

Victoria E Huang

Matthew G Boston

David E Estell

Bradley A Paulson

Jonathan K Lassila

Casper Vroeman

Katarzyna Bell-Rusiewicz

Marc Kolkmam

Karsten Kraugh

Jonathan Lassila

Luis G Cascāo-Pereira

Sura H Hadi

Maguerite A Cervin

Manasi Bhate

Patricia Tran

Hon Kit Chan

Dina Finan

James T Kellis

Bradley Paulson

Karsten M Kragh

William A Cuevas

William Cuevas

Marc Kolman

Sura Hussain Hadi

Bradley Alan Paulson

Brian Edward Jones

Neelem S Amin

Victoria Huang

An automatic dishwashing cleaning composition comprising a variant α-amylase wherein the variant α-amylase comprises amino acid substitution(s) selected from the group consisting of:

Automatic dishwashing detergent composition comprising an amylase

Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.

Alpha-amylase variants

&#x3b1;-Amylase variants

The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.

Perhydrolase

Alpha-amylases from(AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.

[object Object]

An &#x3b1;-amylase from(AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.

Described are variants of alpha (&#x3b1;)-amylases having altered starch hydrolysis profiles. The variants have improved thermostability and increased specific activity, resulting in reduced peak viscosity and altered final viscosity during starch liquefaction. The amylase variants are useful, e.g., in liquefaction and other starch degradation processes.

Variants of a Bacillus strearothermophilus alpha-amylase and uses thereof

Variants ofsp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased thermostability, reduced calcium dependence, increased washing/cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.

Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.

Alpha-amylase variants with altered properties

Variants ofsp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing/cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.

Described are variants of a parent &#x3b1;-amylase that exhibits an alteration in at least one of the following properties relative to said parent &#x3b1;-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, Ca2+ dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.

Disclosed are compositions comprising variants of a parentalpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.

Growing community of inventors

Sunnyvale, CA, United States of America

Sandra W Ramer