Applied Biosystems, LLC

Applera Corporation

Pe Corporation (ny)

Sandra Spurgeon

Linda G Lee

Barnett B Rosenblum

Scott C Benson

Ronald J Graham

Peter Ma

Robert Ruhfel

Kathleen M Perry

Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure RZC(O)RRwhere Ris a Calkyl attached to the donor dye, C(O) is a carbonyl group, Zis either NH, sulfur or oxygen, Ris a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and Rincludes a functional group which attaches the linker to the acceptor dye.

Oligonucleotides and analogs labeled with energy transfer dyes

Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure RZC(O)RRwhere Ris a Calkyl attached to the donor dye, C(O) is a carbonyl group, Zis either NH, sulfur or oxygen, Ris a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and Rincludes a functional group which attaches the linker to the acceptor dye.

Methods of labeling polynucleotides with energy transfer dyes

A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures wherein R-Rare hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or, when taken together, Rand Ris benzo, or, when taken together, Rand Ris benzo; R-R, R-Rand Rmay be hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group; Rand Rmay be hydrogen, lower alkyl, lower alkene, lower alkyne, phenyl, aryl, linking group; Y-Yare hydrogen, lower alkyl, or cycloalkyl, or, when taken together, Yand R, Yand RYand R, and/or Yand Ris propano, ethano, or substituted forms thereof; and X-Xtaken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

4,7-dichlororhodamine dyes labeled polynucleotides

Methods of analyzing polynucleotides employing energy transfer dyes

Kits useful for sequencing nucleic acids

Methods of labeling nucleic acids with energy transfer dyes

A dry composition comprising a nucleotide linked to a fluorescent dye is provided. A method of making a dry composition comprising the step of drying a solution comprising a nucleotide linked to a fluorescent dye is provided. A method is provided for performing a reaction on a nucleic acid substrate comprising: (a) providing a vessel containing a dried composition comprising a nucleotide linked to a fluorescent dye, and (b) adding to the vessel an aqueous solution and the nucleic acid substrate. A kit is provided for determining the sequence of a nucleic acid, the kit comprising a dry composition comprising a nucleotide linked to a fluorescent dye.

Method for drying dye-terminator sequencing reagents

Regents labeled with energy transfer dyes

Energy transfer dyes with enhanced fluorescence

Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R Z C(O)R R where R is a C alkyl attached to the donor dye, C(O) is a carbonyl group, Z is either NH, sulfur or oxygen, R is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R includes a functional group which attaches the linker to the acceptor dye.

Method for detecting oligonucleotides using energy transfer dyes with long stoke shift

Growing community of inventors

San Mateo, CA, United States of America

Sandra Spurgeon