Dow Agrosciences LLC

Corteva Agriscience LLC

Pioneer Hi-bred International, Inc.

Kumar, Sandeep

Alabed, Diaa

Gupta, Manju

Sandeep Kumar

Manju Gupta

Terry R Wright

Diaa Alabed

Andrew Asberry

Andrew F Worden

Andrew J Bowling

Heather Pence

Daren Hemingway

Susan M Jayne

Doug A Smith

Wei Chen

Marcelo Ariel German

Stephen N Novak

Sara Bennett

Carla Ausmus

Carla Nanette Yerkes

Andrew E Robinson

Todd P Glancy

Shreedharan Sriram

Ryan M Lee

Pierluigi Barone

Emily Etchison

Cory M Larsen

Jeffrey Beringer

Tristan E Coram

Nagesh Sardesai

Ronnie Hampton, Jr

Po-Hao Wang

Alicia Walker

Staci Weaver

David Richard Corbin

William James Gordon-Kamm

German Carlos Spangenberg

Donald J Merlo

Steven Robert Webb

Joshua K Young

Zhenglin Hou

Dayakar Reddy Pareddy

Navin Elango

Matthew J Henry

Steven Lee Evans

John White Forster

Noel Cogan

Matthew Hayden

Zhan-bin Liu

Tim Sawbridge

John Mason

Toby Cicak

Huixia Wu

Karthik Narayna Muthuraman

Ajith Anand

Heather Leigh Robinson

W Mike Ainley

Sergi Svitashev

Pohao Wang

Steven L Evans

John Forster

This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in seed tissues of a dicot plant.

Modulation of transgene expression in plants

Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, via the use of a polynucleotide comprising sequences homologous to the target site. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a polynucleotide repair template.

Methods and compositions for homology directed repair of double strand breaks in plant cell genomes

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from aegg cell gene. Some embodiments relate to a promoter or a 5' UTR from aegg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR or a terminator from aegg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

3'UTR sequence for transgene expression

Novel engineered non-natural deaminases are provided. Methods and compositions are provided for modifying the genome of a cell, including a plant cell. Genome modification through guided CRISPR polypeptides and multiple deaminases are provided. Engineered non-natural novel deaminases, including adenosine deaminases are provided. These novel deaminases have improved activity in cells including plant cells.

Engineered deaminases

Disclosed herein are methods and compositions for the repair of site specific nuclease binding sites by targeted integration and/or targeted excision of one or more sequences into a cell.

Reconstruction of site specific nuclease binding sites

The current disclosure relates to methods and compositions for improving plant varieties through plant breeding and plant genetics. For instance, the disclosure concerns increasing the recombination frequency of a heterozygous trait genetically linked to a second trait within plants. Further, the disclosure concerns breaking the genetic linkage between a first allele and a second allele.

Methodologies and compositions for creating targeted recombination and breaking linkage between traits

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from aegg cell gene. Some embodiments relate to a promoter or a 5' UTR from aegg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3&#x2032; UTR or a terminator from aegg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Plant promoter for transgene expression

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing aGRMZM2G144030 promoter. Some embodiments relate to aGRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to aGRMZM2G144030 3'UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

Plant promoter and 3'UTR for transgene expression

This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in male plant tissues, for example, to provide an economical male sterility system of hybrid seed production.

Tissue-specific expression and hybrid plant production

An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5' and 3&#x2032; ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Engineered transgene integration platform (ETIP) for gene targeting and trait stacking

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a(Pavir.J00490) egg cell gene. Some embodiments relate to a promoter from a(Pavir.J00490) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3' UTR from a(Pavir.J00490) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Plant promoter from

Growing community of inventors

Carmel, IN, United States of America

Sandeep Kumar