Perseptive Biosystems Inc.

Purdue Research Foundation

Sandeep K Paliwal

Noubar Boghos Afeyan

Fred E Regnier

Satish Jindal

David Evans

Kevin Williams

Aruna Pingali

Laszlo Varady

Fred B Regnier

Timothy K Nadler

The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second &ldquo;dimension&rdquo;. The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.

High speed, automated, continuous flow, multi-dimensional molecular selection and analysis

Disclosed are specifically designed binding, 'protein imaged' sorbents which reversibly bind with high specificity and affinity a preselected macromolecule, specifically a protein. The sorbents define one or more cavities which have a binding surface complementary in shape to the molecular surface of the macromolecule and a plurality of positively and negatively charged chemical moieties spatially distributed in a mirror image and charge inverse of a subset of the ionizable groups on the molecular surface of the macromolecule. Also disclosed are methods of producing such sorbents, useful over a range of conditions, for both preparative and analytical chromatographic separations or for use in various types of analyses.

Protein imaging

Growing community of inventors

Mountain View, CA, United States of America

Sandeep K Paliwal