University of Texas System

Sanchita Bhadra

Andrew D Ellington

Jimmy Gollihar

Raghav Shroff

Yu Sherry Jiang

Randy Allen Hughes

Bingling Li

Yan Du

Eric V Anslyn

Jagannath Swaminathan

Jared Ellefson

Alexander Boulgakov

Edward Marcotte

Michelle Byrom

Arti Pothukuchy

Daniel Garry

Brendan Floyd

Sheng Cai

Jon Laurent

Erhu Xiong

Cheulhee Jung

John N Milligan

The present disclosure provides methods for molecular neighborhood detection of molecules, such as by iterative proximity ligation or split-and-pool methods for obtaining positional information.

Molecular neighborhood detection by oligonucleotides

Disclosed are methods for isothermal nucleic acid amplification and detection.

Strand displacement with loop-mediated isothermal amplification

Disclosed herein is a method of utilizing an enzyme in a nucleic acid manipulation process, the method comprising: a) transforming a microorganism with a non-native enzyme; b) inducing expression of the enzyme in the microorganism, thereby producing the non-native enzyme; c) adding the microorganism of step b) directly to a non-naturally occurring nucleic acid manipulation process, wherein the non-native enzyme is not purified from the microorganism prior to addition to the nucleic acid manipulation process; and carrying out the nucleic acid manipulation process using the enzyme. Importantly, this method can be carried out without the need to purify the enzyme from the cell producing it before it is used in the nucleic acid manipulation method. Also disclosed herein is a kit for carrying out a nucleic acid manipulation process, the kit comprising a) a microorganism expressing a non-native enzyme; b) nucleic acids of interest; and c) reagents for use in the nucleic acid manipulation process.

Methods and kits for using recombinant microorganisms as direct reagents in biological applications

Disclosed are compositions and methods for nucleic acid amplification and detection. Specifically, disclosed herein are compositions and methods that allow for amplification of nucleic acids at a wide variety of temperatures. This includes a polymerase which is thermostable at high temperatures, and a method of amplification that can be conducted at relatively low temperatures.

Methods and devices related to amplifying nucleic acid at a variety of temperatures

Disclosed are compositions and methods for isothermal nucleic acid amplification and detection.

Methods and devices related to toehold-based strand displacement with loop-mediated isothermal amplification

Growing community of inventors

Austin, TX, United States of America

Sanchita Bhadra