Corbion Biotech, Inc.

Roquette Freres

Solazyme Roquette Nutritionals, LLC

Samuel Patinier

Amandine Druon

Philippe Looten

John Piechocki

Michel Perrut

Leslie M Norris

Marie Le Ruyet

Ruchir Desai

Enrique Baliu

Heike Jerosch

Damien Passé

Philippe Lefevre

Damien Passe

José Lis

David Segard

Eric Francais

Beatrice Toursel

Dan Dueppen

Francois Delannoy

Marilyne Guillemant

Béatrice Toursel

Vincent Perrut

Aurore Satre-Buisson

Marilynne Guillemant

François Delannoy

Maryline Guillemant

Thus, the present invention relates to a method for determining the organoleptic quality of a microalgal flour composition, comprising determining the total content of 13 volatile organic compounds, the 13 volatile organic compounds being heptanal, 3-octen-2-one, 2,4-heptadienal, 3,5-octadien-2-one, 2,4-nonadienal, 2,4-decadienal, hexanoic acid, 2-ethylhexanoic acid, heptanoic acid, myristate-1, laurate-1, myristate-2 and geranyl acetone.

Microalgal flour compositions of optimised sensory quality

The invention relates to a method for preparing a protein isolate of the biomass of microalgae of the genuscharacterised in that it comprises the following steps: supplying a microalgal biomass produced by fermentation, washing the biomass so as to eliminate the soluble interstitial compounds and concentrating the biomass, mechanically grinding the washed and concentrated biomass in a horizontal ball grinder-type system in order to produce an emulsion, destructuring the emulsion thus produced, triple-phase separation so as to separate the soluble fraction from the fractions containing the lipids and the cell debris, recovery of the soluble fraction thus produced in order to produce the soluble protein isolate, then evaporation, pasteurisation and atomisation of said protein isolate.

Method for extracting soluble proteins from microalgal biomass

A method for preparing a protein isolate of the biomass of microalgae of the genus, includes the following steps: supplying a microalgal biomass produced by fermentation, washing the biomass so as to eliminate the soluble interstitial compounds and concentrating the biomass, mechanically grinding the washed and concentrated biomass in a horizontal ball grinder-type system in order to produce an emulsion, destructuring the emulsion thus produced, triple-phase separation so as to separate the soluble fraction from the fractions containing the lipids and the cell debris, recovery of the soluble fraction thus produced in order to produce the soluble protein isolate, then evaporation, pasteurization and atomization of the protein isolate.

The present invention relates to a method for optimising the downstream processing of a protein-rich microalgae biomass of thegenus previously prepared by fermentation in heterotrophic conditions and in the absence of light, comprising: 1) providing biomass comprising more than 50% protein by dry weight of biomass; next, at low temperature, carrying out the following steps: 2) harvesting the biomass at the end of fermentation, 3) washing and concentrating the biomass, 4) optionally, lysing the biomass, next, without low temperature stress, 5) optionally, concentrating the biomass slurry, 6) applying heat treatment, 7) drying the biomass obtained in this way in order to obtain the product, a step of adjusting the pH to 7 being applied before or after the heat treatment step 6).

Method for optimizing the production efficiency, organoleptic quality and stability over time of a protein-rich microalgae biomass

Disclosed is a method of mechanically crushing microalgae cells of the genusat an industrial scale, the mechanical crushing being carried out in a horizontal ball mill system. In the method, the balls have an apparent density of between 2 and 3.5 kg/l, the filling rate of the crushing chamber is greater than or equal to 80%, and preferably greater than or equal to 85%, and the mechanical crushing is carried out continuously by a series of successive passes.

Optimised method for breaking chlorella walls by mechanical crushing

An agitator shaft () for a grinding mill, including: &#x2014;an axle () intended to be driven in rotation, &#x2014;a succession of flat agitator elements () and tubular spacers () mounted in alternation on the axle and in a stack along the axle (), &#x2014;end stops (), secured to the axle, that keep the stack of agitator elements and spacers under compression, characterized in that the tubular spacers () have orifices allowing the free circulation of material and fluids from the outside of the agitator shaft to the inside, and vice versa.

Agitator shaft for a grinding mill

The present invention relates to microalgal food products with acceptable sensory characteristics and methods of producing the food products. The flour can be produced by cultivating microalgal cells of a strain ofunder conditions of acceptable pH and dissolved oxygen to produce a desired amount of lipid. The microalgal cells can be lysed, heat-treated, washed and dried to produce a microalgal flour that can be incorporated into a variety of products.

Microalgal flour

The invention relates to a method for determining the organoleptic quality of a protein-rich microalgal biomass composition, comprising the determination of the content of 11 volatile organic compounds, wherein the 11 volatile organic compounds are pentanal, hexanal, 1-octen-3-ol, 2-pentylfuran, octanal, 3,5-octadien-2-ol (or 3-octen-2-one), 3,5-octadien-2-one, nonanal, 2-no-nenal, (E,E)-2,4-nonadienal and hexanoic acid.

Protein-rich microalgal biomass compositions of optimized sensory quality

The invention relates to a method for preparing a composition that is very rich in squalene produced by fermentation of micro-organisms. The method is characterized in that it comprises a purification step selected from the group including: supercritical CO2 extraction in a multi-stage counter-current fractionation column with extract reflux, and short-path molecular distillation.

Method for refining squalene produced by microalgae

The invention relates to a method for preparing a composition rich in lutein produced by microalgae, particularly microalgae belonging to thegenus, more specifically produced by, characterized in that said method includes the sequence of the following steps: 1) preparing a cell lysate from the microalgae biomass; 2) treating the lysed microalgae biomass with a polar solvent, preferably ethyl acetate, in order to obtain an oleoresin containing the lutein and the lipids from the initial biomass; 3) extracting the oleoresin obtained in step 2) by means of a non-polar solvent, here a fluid at supercritical pressure, in order to obtain a fraction rich in non-polar lipids, mainly consisting of triglycerides, and an insoluble fraction rich in lutein; and 4) recovering the thus lutein-enriched fraction.

Method for preparing a composition rich in lutein produced by microalgae

The invention concerns a method of preparing an oil enriched with ethyl esters of DHA produced by fermenting microorganisms, characterized in that it comprises a step of purification by so-called 'short path' molecular distillation.

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Quesnoy-sur-Deule, France

Samuel Patinier