Fmc Corporation

Other

Pall Corporation

Valentis, Inc.

Samuel Nochumson

Richard B Provonchee

Francis H Kirkpatrick

Jonathan H Morgan

Ross Durland

Rexford Hayes

John Welp

Audrey Yu-Speight

Hassan Ait-Haddou

Bruce S Goldberg

Frank Onyemauwa

Donald Walter Renn

Richard B Cook

Kenneth B Guiseley

William C Snow

Henry J Witt

Foner P Curtis

Peter Levison

Kuoewi Wu

Kuowei Wu

Jonathan Haigh

David L Harper

Mikhail V Ostrovsky

Kenneth Guiseley

Substrates comprising a solid support, a ligand, and a linker comprising at least one C, O, N, or S atom covalently connecting the solid support to the ligand, are disclosed, along with methods of using and making the substrates, and devices including the substrates.

Mixed mode ligands

A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinantcells. Effective, controllable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and neutralization.

Process and equipment for plasmid purification

Methods for testing the chromatography type and/or the integrity of a chromatography membrane or monolith, preferably, for testing the chromatography type and integrity of a chromatography device comprising a chromatography membrane or chromatography monolith while the membrane or monolith is sealed in a housing, are disclosed.

System and method for testing chromatography media and devices

A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinantcell. Effective, contgrollable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and nuetralization.

Disclosed is a centrifuge tube containing a porous selection means for selectively separating and recovering biological substances from gels, broths, or solutions containing the same, and thus concentrating them, the tube comprising separate upper and lower containers and means for joining the two containers to form a unitary device, the bottom of the upper container comprising a porous selection means, preferably a membrane containing a particulate substance which will selectively bind the biological substances while allowing unbound substances to pass through from the upper to lower container while being centrifuged. A method for recovering the biological substances in high concentration using the centrifuge tube is also provided, comprising introducing said gels, broths, or solutions containing the desired biological substances, centrifuging the same until the desired substance is bound to the selection means, and eluting the bound material from the selection means to recover the substance in concentrated form.

Method for separation and recovery of biological materials

A nucleic acid sequencing gel comprising 2 to 10% of a gelling polysaccharide, preferably agarose; a denaturing agent, a non-gelling additive, preferably glycerol, and electrophoretic buffers. This composition is capable of resolving nucleic acid fragments having at least 50 bases and which differ from each other by at least one base.

Agarose compositions for nucleic acid sequencing

Polysaccharide resolving gels and gel systems for stacking

A method for forming agarose beads, which may incorporate biological material at least partially labile at above 40.degree. C., in which an aqueous agarose solution is formed into bead-size portions which are gelled by contacting the portions with a cooled atmosphere, gas, and/or smooth hydrophobic surface, and then collected.

Agarose beads, preferably incorporating biological materials

Purified agarose suitable for rapid electrophoresis, characterized by a sulfate content of less than 0.2 wt % but greater than zero, a pyruvate content of 0-0.1 wt %, and a nitrogen content of 0-0.02 wt %. Gels prepared from the agarose exhibit a gel strength at 1.0 wt % concentration of at least 1200 g/cm.sup.2, substantial absence of DNA binding in 0.07 M or less tris acetate buffer, and an electroendosmosis (EEO) at 1.0 wt % concentration of 0.05 or less. Agaroses are purified to provide the low EEO material by dissolving agarose or alkali-modified agar in an aqueous medium buffered at a pH of 6.0 to 8.0 and containing no more than 2.0 nM salt as chloride, and precipitating the agarose by contact with a lower alkanol.

High gel strength low electroendosmosis agarose

An electrophoresis medium, comprising a copolymer of acrylamide and an ethylenically unsaturated resin formed by replacing at least some of the hydroxyl hydrogens in a polysaccharide with an ethylenically unsaturated group, is described.

Polyacrylamide cross-linked with a polysaccharide resin as

An electrophoretic support comprising a base plate having on at least one side thereof, a layer of an adherent resin containing ethylenically unsaturated groups capable of undergoing copolymerization with acrylamide. The support provides excellent adhesion for polyacrylamide gels and other electrophoretic media such as agarose.

Growing community of inventors

Randolph, NJ, United States of America

Samuel Nochumson