University of Pittsburgh

Samuel Aaron Tisherman

S William Stezoski

Patrick Kochanek

Xianren Wu

Lyn Yaffe

Provided are methods for inducing preservation in a patient and resuscitating that patient. At least three hours of preservation, with successful resuscitation, are realized using the methods described herein. The methods involve flushing a patient with an oxygenated, cold-flush solution, such as normal saline, having an energy source. The patient may be cooled to deep or profound hypothermia to induce preservation. The patient may be resuscitated by warming to from about 33&#xb0; C. to about 36&#xb0; C., and then may be slowly warmed over a 24 to 72 hour period to normothermia. Kits for inducing preservation also are provided.

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Pittsburgh, PA, United States of America

Samuel Aaron Tisherman