Cytiva Sweden Ab

Sameera Simha Hindupur

Klaus Gebauer

Prashanth Hosabettu Mohan

Manoj Kumar Ramakrishna

Andreas Castan

Ajit S Vernekar

Nachiket Karmarkar

Sasi Kumar Nutalapati

Swapnil Puranik

Nityananda Maiya

Sasikumar Arumugam

Sudheer Mygapula

Bashkar Hv

Shashidhar Hedge

The invention discloses a method of controlling a transmembrane pressure (TMP) in a crossflow filtration apparatus, comprising the steps of: a) providing a crossflow filtration apparatus comprising a pump () fluidically connected via a retentate compartment () of a crossflow filter () and a length of flexible tubing to a regulator valve (1; 101) acting on the flexible tubing, wherein the regulator valve is a high precision pinch valve. The apparatus further comprises a first pressure transducer () adapted to measure a pressure between the retentate compartment and the regulator valve, a second pressure transducer () adapted to measure a pressure between the pump and the retentate compartment and a third pressure transducer () adapted to measure a pressure at a permeate outlet () of the crossflow filter, and a control unit () electrically or electromagnetically connected to said regulator valve, the first, second and third pressure transducers and optionally to the pump; b) pumping a liquid with the pump via the second pressure transducer, through the retentate compartment via the length of flexible tubing, the first pressure transducer and the regulator valve; c) controlling the regulator valve with the control unit, such that a TMP calculated by formula (I) TMP=(P+P)/2−Pcorresponds to a predetermined TMP value or to an interval between two predetermined TMP values, wherein Pis a pressure reading from the second pressure transducer, Pis a pressure reading from the first pressure transducer and Pis a pressure reading from the third pressure transducer.

Method for pressure control in crossflow filtration

The present invention relates to a computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container (BR), the method comprising welding () a vial to a connector to obtain a fluid-tight seal between the vial (V) and a connector (C), the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial, sealing () an opposite end of one of the conduits, generating () a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit, measuring () a fluid pressure within at least one of the conduits, determining () a fluid pressure change based on the ambient pressure and the measured fluid pressure, determining () the weld integrity test result as pass or as fail based on the fluid pressure change.

Method for determining a weld integrity test result

Growing community of inventors

Karnataka, India

Sameera Simha Hindupur