Bioresponse, LLC

Other

Baxter International Inc.

Baxter Healthcare Inc.

Sam Rose

Glenn C Buchanan

Sterile interconnection between disassociated segments of thermostable, compressible fluid conduit tubing is achieved by arranging within the bore of the free end of each segment a conductive hollow metal tube so as to establish a connected fluid flow path from one segment to the other, through the metal tube, and then sterilizing the connection by induction heating.

Method for making sterile connections between fluid conduit tubes

Cells producing a desired product or products, such as antibodies, are cultured with a culture medium comprising lymph. The lymph is continuously flowed across a semi-permeable membrane, on the other side of which are the cells to be cultured. The membrane is sized so as to retain cells, and their desired products, on one side of the membrane while permitting components of the lymph-containing culture medium (flowing across the other side of the membrane) to pass through the membrane to provide nutrition, etc. to the culturing cells.

Method for in vitro culture of mammalian cells

The invention relates to techniques for isolating from a mixed population of cells disired living cells either producing and releasing a particular product or having a characteristic molecule on their surface. The isolation techniques depend upon the localized interaction between the product (or molecule) and other agents added to the system such that distinguishable conditions can be caused to occur (or not occur) only in the immediate vicinity of desired cells which produced and released the product or which contain the molecule on their surface.

Method for isolating product-producing cells

Disclosed herein is a process for the in vitro culturing of cells or tissues such that the cells or tissues grow, behave and function in substantially the manner such cells or tissues grow, behave and function within the body, wherein the cells or tissues are contacted or bathed with fresh flowing cell-free lymph. The cell-free lymph may be obtained directly from a living host by removing lymph from the host, rendering the lymph cell-free by suitable means and then using the cell-free lymph as the culture medium for cells or tissues of either the same or different species as that of the host. The cells or tissues preferably are contacted with the cell-free lymph in a chamber in which the cells or tissues are exposed either directly to the flowing lymph or to components thereof.

Method for culturing cells or tissues

A process for the removal of a specific antibody from the lymph fluid free of lymph cells is disclosed. The lymph is taken from a subject (e.g., human or animal) by means of a thoracic duct fistula by raising the pressure of the central venous system in order that the pressure is above the atmospheric pressure of the thoracic duct. Lymph is separated into cells and lymph fluid containing antibodies. Cells are returned to the subject intravenously. Lymph fluid is mixed with substrate particles attached with specific antigens. An antibody-antigen-substrate particle complex is formed and removed. Subsequently, specific antibody can be split from the specific antigens attached to a substrate particle. The antigen-attached substrate particles can then be re-used. Specific antibody can be introduced into another subject or used for other purposes in biology, in chemistry, and in veterinary and clinical medicine.

Method for the continuous removal of a specific antibody from the lymph

The disclosed signalling apparatus provides a different identifying signal in response to a ringing signal for one or more telephone station sets connected by a common line to a centralized switching, control and signalling machine. The different identifying signals are generated in response to the number and timing of ringing signals applied to the line. If a first ring is sensed and within a predetermined first time a second ring is sensed, a first identifying signal is initiated. If a first ring is sensed and after the predetermined first time but before a predetermined second time a second ring is sensed, a second identifying signal is initiated. If a first ring is sensed, but a second ring is not sensed before the predetermined second time, the signalling apparatus is reset to initial conditions. In the disclosed preferred embodiments of the invention, the second identifying signal may be used to provide a signal to a second telephone connected to the common telephone line, or it may be used to control the operation of a remote appliance.

Telephone signalling method and apparatus

A process for augmenting production of a specific antibody from a patient including the steps of administering a specific antigen to the patient to cause production of the specific antibody, or choosing a subject containing an antigen, e.g., cancer, or one already immunized or making antibodies to an endogenous or exogenous antigen, performing a thoracic duct fistula on the patient, raising the central venous pressure of the patient for example, by pericordial tamponde, and collecting lymph from the fistula, centrifuging the collected lymph to separate the lymph cells therein from the lymph fluid, and to form a thin elongated layer of the separated cells, treating the thin elongated layer of lymph cells to assure that they are substantially free of the specific antibody produced, dispersing the cells in a physiologically balanced saline solution and returning the dispersed cells and solution to the patient intravascularly, and giving appropriate replacement therapy to maintain normal control and health of all other systems in the body, the replacement therapy comprising fluids which can be of several kinds, but having in common that they are substantially free of the specific antibody.

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Eggertsville, NY, United States of America

Sam Rose