Applied Biosystems, LLC

The Perkin-Elmer Corporation

Life Technologies Corporation

Applera Corporation

Other

Zoecon Corporation

Biometric Imaging, Inc.

Applera Coporation

Sam L Woo

Steven Fung

Linda G Lee

Steven Menchen

Paul D Grossman

Emily S Winn-Deen

Richard T Reel

David Cox

Charles R Connell

Willy K Wiyatno

Mark F Oldham

Vergine Chakerian

N Davis Hershey

Hongye Sun

Peter Ma

Dennis Lehto

Janusz B Wojtowicz

Douglas Greiner

Jon M Sorenson

Congcong Ma

Benjamin G Schroeder

Lloyd M Smith

James C Nurse

Jing Tian

Ronald J Graham

Jim Nurse

Clive A Henrick

Richard P Haugland

Jeffrey N Labovitz

Michael M Leippe

Ben F Johnson

B John Bergot

Peter Nien-Tung Ma

Ron Graham

Dennis Letho

J Scott Eadie

Emily S Win-Deen

David M Cox

Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.

Apparatus, system, and method using immiscible-fluid-discrete-volumes

A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

Chemically-enhanced primer compositions, methods and kits

A system may include a first conduit configured to form a first batch of discrete volumes of aqueous fluid separated by spacing liquid disposed between consecutive volumes of aqueous fluid, the spacing liquid being immiscible with the aqueous fluid volumes; a second conduit, fluidically coupled to the first conduit, the second conduit configured to statically hold the first batch of discrete volumes of aqueous fluid; and a third conduit configured to receive the first batch of discrete volumes of aqueous fluid from the second conduit. The third conduit can be configured to transfer the discrete volumes of aqueous fluid of the first batch for downstream processing.

Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other

A method for sequencing a nucleic acid template includes forming a nanowire assembly including a semiconductor nanowire and a probe covalently bound to the semiconductor nanowire; contacting the nanowire assembly with a template nucleic acid; contacting the nucleic acid duplexes with an extension nucleic acid, the extension nucleic acid joined to the probe; disrupting the nucleic acid duplexes; and measuring an electrical characteristic of a nanowire assembly of the set of nanowire assemblies.

Nanowire-based system for analysis of nucleic acids

A method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The discrete volumes are separated from one another by a liquid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.

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Redwood City, CA, United States of America

Sam L Woo