University of Maine System

The Jackson Laboratory

University of Utah Research Foundation

Sam Hess

Joerg Bewersdorf

Travis J Gould

Manuel F Juette

Erik Jorgensen

Michael Darin Mason

Brian Thomas Bennett

Mudalige Siyath Gunewardene

A system () and method for creating three dimensional images using probe molecules is disclosed and described. A sample is mounted on a stage (). The sample has a plurality of probe molecules. The sample is illuminated with light, causing the probe molecules to luminesce. The probe luminescence can be split into at least four paths corresponding to at least four detection planes corresponding to object planes in the sample. The at least four detection planes are detected via a camera (). Object planes in corresponding recorded regions of interest are recorded in the camera (). A signal from the regions of interest is combined into a three dimensional image.

Microscopy system and method for creating three dimensional images using probe molecules

A method of performing 3D photoactivation microscope imaging includes providing a sample having a plurality of probes, each of the plurality of probes including a photo-activatable material. Probes from the plurality of probes are activated to form a sparse subset of probes, the sparse subset of probes having probes that are spatially separated by at least a microscope resolution. The sample is illuminated with a readout light source, and light emitted from activated probes is detected. Based on the light emission detected from the activated probes, localized three-dimensional positions of the activated probes are obtained.

Microscopic imaging techniques

A microscopy system is configured for creating 3D images from individually localized probe molecules. The microscopy system includes a sample stage, an activation light source, a readout light source, a beam splitting device, at least one camera, and a controller. The activation light source activates probes of at least one probe subset of photo-sensitive luminescent probes, and the readout light source causes luminescence light from the activated probes. The beam splitting device splits the luminescence light into at least two paths to create at least two detection planes that correspond to the same or different number of object planes of the sample. The camera detects simultaneously the at least two detection planes, the number of object planes being represented in the camera by the same number of recorded regions of interest. The controller is programmable to combine a signal from the regions of interest into a 3D data.

3D biplane microscopy

Growing community of inventors

Stillwater, ME, United States of America

Sam Hess