Centre National De La Recherche Scientifique

Université´ D'aix-Marseille

Basf Se Corporation

Université Paris—sud

Université Paris-Saclay

Sam Dukan

Boris Vauzeilles

Jordi Mas Pons

Aurélie Baron

Adrien Ducret

Monzer Awwad

Yannick Fovet

Audrey Dumont

Annie Malleron

Aurelie Baron

Marina Periame

The invention relates to a method for labeling specifically living bacteria, comprising the steps of: a) incubating said bacteria of said sample with at least one modified monosaccharide compound comprising a first reactive chemical group capable to chemically react with a second reactive group, so that a residue bearing said first reactive group is incorporated into the envelope of such bacteria, and b) contacting said modified monosaccharide residue incorporated within the envelope of the bacteria with a labeling molecule comprising a said second reactive group, for generating the chemical reaction of said first reactive group with said second reactive group, characterized in that said modified monosaccharide compound has the following formula (I), or a salt thereof: Wherein &#x2014;A, B and C can be independently H, OH, NH, OH and NHbeing substituted or not by protecting groups thereof and &#x2014;D is an alkyl chain in Cto C, each carbon being substituted or not by OH or NH, OH and NHbeing substituted or not by protecting groups thereof and &#x2014;at least one of A, B, C or D groups is substituted by a said first reactive group. 

Method for labeling specifically living bacteria comprising the use of modified non endogenous monosaccharide compounds

The invention relates to a method for labeling specifically living microorganisms in a sample comprising microorganisms, the method comprising the steps of: a) incubating said microorganisms of said sample with at least one modified monosaccharide compound comprising a first reactive chemical group capable to chemically react with a second reactive group, so that a residue bearing said first reactive group is incorporated into such microorganisms, and b) contacting said residue incorporated in the microorganisms, with a labeling molecule comprising a said second reactive group, for generating the chemical reaction of said first reactive group of said residue incorporated within said living microorganisms with said second reactive group of said labeling molecule, resulting in a covalent link, characterized in that the said modified monosaccharide compound has the following formula (I'), or a salt thereof: &#x2014;X can be O, NH or S, preferably O and NH, and &#x2014;R1 and R2 can be independently H, OH, NH, OH and NHbeing substituted or not by protecting groups thereof, preferably substituted by alkyl, hydroxyalkyl, acyl, formyl or imidoyl groups, and &#x2014;R3 is H or an alkyl chain in Cto C, each carbon being substituted or not substituted by OH or NHsubstituted or not by protecting groups thereof, preferably by alkyl, hydroxyalkyl, acyl, formyl or imidoyl groups, and &#x2014;at least one of X, R1, R2 and R3 groups, preferably R3, being substituted by a said first reactive group Ra. 

Method for labeling specifically living microorganisms comprising the use of modified monosaccharide compounds

A kit for labeling bacteria, the kit including:

Kit for labelling bacteria

The invention relates to a method for labeling specifically living bacteria of a given category of Gram negative bacteria in a sample comprising bacteria, the method including the steps of:

Method for labeling specifically living and bacteria comprising the use of modified monosaccharide compounds

The present invention concerns a method for labeling specifically living bacteria of a given category in a sample, the method including: a) incubating said bacteria of said sample with at least one analog of a monosaccharide compound, and b) contacting the bacteria with a labeling molecule having a second reactive group.

Method for specifically labeling living bacteria

A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms (1) contacting said microorganisms of said sample with at least one repair compound and a growth medium, and (2) incubating the product of steps (1), and (3) detecting and quantifying said viable microorganisms, in which the microorganisms are of the species, and in which the repair compound directly or indirectly causes an effect on the metabolism to reduce the oxidative stress of the microorganism. The invention also includes a kit for more accurately detecting and enumerating viable microorganisms of the speciesin a sample suspected of containing said microorganisms.

Detection and enumeration of microorganisms

A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms: (1) contacting said microorganisms of said sample with repair compounds and a growth medium, and (2) incubating the product of step (1),and (3) detecting and enumerating said microorganisms, in which the microorganisms are of the species, and in which the repair compounds comprise: (a)serine;(b)threonine;(c)a compound containing calcium ions at a dose of 10to 10mM;(d)a compound containing magnesium ions at a dose of 10to 10mM;(e)a compound containing potassium ions;(f) glutamic acid or a salt thereof; and (g)pyruvic acid or a salt thereof. The invention also discloses a kit for detecting and enumerating viable microorganisms of the speciesin a sample suspected of containing said microorganisms.

Growing community of inventors

Marseilles, France

Sam Dukan