Sloan-kettering Institute for Cancer Research

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Universita' Degli Studi Di Catanzaro “Magna Graecia”

Salvatore Venuta

Karl Welte

Roland Mertelsmann

Maria Caterina Turco

Antonello Petrella

Rita Bisogni

Maria Fiammetta Romano

Annalisa Lamberti

It is described a nucleotide sequence and a corresponding protein for which a role and utility in the regulation of programmed cell death or apoptosis are defined. Applications and uses of the sequence and corresponding protein concern all the physiologic and pathologic processes that involve cell death (neoplasias, degenerative diseases, tissue infarcts, autoimmune diseases, differentiation processes, etc.).

Nucleotide sequences and protein(s) encoded by such nucleotides for modulation of apoptosis

This invention provides a composition comprising a therapeutically effective amount of purified human interleukin-2 and a pharmaceutically acceptable carrier.

Highly purified interleukin-2 and method

Interleukin 2 (IL 2; T-cell growth factor), produced with and without costimulation by Burkitt's lymphoma line Daudi, is highly purified approximately over 37,000-fold to apparent homogeneity from lymphocyte-conditioned medium derived from normal human blood cells by (NH.sub.4).sub.2 SO.sub.4 -precipitation, ion-exchange chromatogr

Purified interleukin 2

Interleukin 2 (IL 2; T-cell growth factor), produced with and without costimulation by Burkitt's lymphoma line Daudi, is highly purified approximately over 37,000-fold to apparent homogeneity from lymphocyte-conditioned medium derived from normal huma

Process for preparing purified interleukin-2

Interleukin 2 (IL 2; T-cell growth factor), produced with and without costimulation by Burkitt's lympho

Uses of interleukin-2

Interleukin 2 (IL 2; T-cell growth factor), produced with and without costimulation by Burkitt's lymphoma line Daudi, is highly purified approximately over 37,000-fold to apparent homogeneity from lymphocyte-conditioned medium derived from normal human blood cells by (NH.sub.4).sub.2 SO.sub.4 -precipitation, ion-exchange chromatography, gel filtration and hydrophobic chromatography. hp IL-2 is free of pyrogens, B cell inducing factor, B cell growth factor, interferon, CSF, and thymocyte differentiating factor. Nature IL 2 produced in the absence of Daudi cells has a molecular weight of about 26,000 daltons as measured by gel filtration and yields IL 2 having two molecular weights of about 16,000 and 17,000 daltons after denaturation as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. IL 2 produced in the presence of Daudi cells shows a molecular weight of approximately 14,500 daltons as measured by both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This highly purified IL 2 is shown to correct immunodeficiency states in vitro and in vivo, especially in human patients. It shows value in the treatment of AIDS and immunodeficiency resulting from chemotherpy of cancer, as well as transplantation disorders such as graft versus host disease.

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Naples, Italy

Salvatore Venuta