Agilent Technologies, Inc.

Hewlett-packard Company

Other

Sally A Swedberg

Laurie S Mittelstadt

Patrick Kaltenbach

Klaus E Witt

Fritz Bek

Reid Alyn Brennen

Hongfeng Yin

Karla M Robotti

Paul K Wolber

Karin Caldwell

Jacqueline M Tso

Dominique M Freeman

Douglass McManigill

James E Young

Thomas P Doherty

John A Chakel

Joseph J Pesek

Jurgen A Lux

A method is provided for preparing high surface-area texturing of a substrate using methods by which material from a substrate is subtracted from or added to the surface of the substrate. In one embodiment, the method is a subtractive lithographic method that involves exposing a laser-ablatable substrate, such as a polymeric or ceramic substrate, to laser light. A mask may be used to define the pattern of light incident on the substrate. High surface-area textured substrates, in particular, miniaturized planar analysis devices having high surface-area textured features, prepared by the methods disclosed herein, are also provided. A method by which the high surface-area textured substrate or the miniaturized planar analysis device is used as a master from which replicate copies thereof may be made is also provided.

Producing a substrate having high surface-area texturing

Methods and compositions for modifying the surface of a polymeric substrate are provided. In the subject methods, at least a portion of the surface of the polymeric substrate is contacted with a biofouling resistant surfactant composition. At least a portion of the surfactant molecules of the composition are end modified with a non-reactive, charged end group that is charge stable over a pH range of about 2 to 12. The subject methods and compositions find particular use with electrophoretic devices, such as capillary electrophoretic devices.

Methods and compositions for modifying the surface of a polymeric substrate

A method is provided for preparing high-surface area texturing of a substrate using methods by which material from a substrate is subtracted from or added to the surface of the substrate. In one embodiment, the method is a subtractive lithographic method that involves exposing a laser-ablatable substrate, such as a polymeric or ceramic substrate, to laser light. A mask may be used to define the pattern of light incident on the substrate. High-surface area textured substrates, in particular, miniaturized planar analysis devices having high-surface area textured features, prepared by the methods disclosed herein are also provided. A method by which the high-surface area textured substrate or the miniaturized planar analysis device is used as a master from which replicate copies thereof may be made is also provided.

Method for producing high-structure area texturing of a substrate, substrates prepared thereby and masks for use therein

Methods and compositions for modifying the surface of a polymeric substrate are provided. In the subject methods, at least a portion of the surface of the polymeric substrate is contacted with a biofouling resistant surfactant composition. At least a portion of the surfactant molecules of the composition are end modified with a non-reactive, charged end group that is charge stable over a pH range of a bout 2 to 12. The subject methods and compositions find particular use with electrophoretic devices, such as capillary electrophoretic devices.

A microanalytical device is provided for conducting chemical processes using small amounts of fluid. The devices include microstructures, e.g., microcavities, microchannels and the like, that are laser ablated or otherwise formed in a support substrate, and can be used in a variety of chemical and biochemical methods, including chromatographic, electrophoretic and electrochromatographic separations, screening and diagnostics, and chemical and biochemical synthesis. The devices are formed from a material that is thermally and chemically stable and resistant to biofouling, significantly reducing electroosmotic flow and unwanted adsorption of solute. Preferred materials are polymeric.

Microanalytical device and use thereof for conducting chemical processes

PCR microreactor for amplifying DNA using microquantities of sample fluid

The present invention relates to microdevices for introducing a small volume of a fluid sample into an ionization chamber. The microdevices are constructed from a substrate having a first and second opposing surfaces, the substrate having a microchannel formed in the first surface, and a cover plate arranged over the first surface, the cover plate in combination with the microchannel defining a conduit for conveying the sample. A sample inlet port is provided in fluid communication with the microchannel, wherein the sample inlet port allows the fluid sample from an external source to be conveyed in a defined sample flow path that travels, in order, through the sample inlet port, the conduit and a sample outlet port and into the ionization chamber. Optionally, the fluid sample undergoes a chemical or biochemical reaction within an integrated portion of the microdevice before reaching the ionization chamber. A nebulizing means nebulizes the fluid sample in a nebulizing region adjacent to the sample outlet port. The invention also relates to a method for introducing a fluid sample using the microdevice.

Integrated sample preparation, separation and introduction microdevice for inductively coupled plasma mass spectrometry

&ldquo;A miniaturized planar device is described for use in a liquid phase analysis system. The device comprises a separation compartment that is in fluidic communication with a make-up flow channel and a channel compartment that terminates in an on-device mass spectrometer delivery means. The device is formed by microfabrication of microstructures in novel support substrates.&rdquo;

Miniaturized device for separating the constituents of a sample and delivering the constituents of the separated sample to a mass spectrometer

A microanalysis device having a plurality of sample processing compartments is described for use in liquid phase analysis. A microanalysis device system, comprising a plurality of interconnected microanalysis devices. The device is formed by microfabrication of microstructures in novel support substrates. The invention herein can be used for the analysis of small and/or macromolecular and/or other solutes in the liquid phase.

Device for high throughput sample processing, analysis and collection, and methods of use thereof

Micro-fluid devices and methods for their use are provided. The subject devices are characterized by the presence of at least one micro-valve comprising a phase reversible material, e.g. a reversible gel, that reversibly changes its physical state in response to an applied stimulus, e.g. a thermoreversible gel. In using the subject device, fluid flow along a flow path of the device is modulated by applying an appropriate stimulus, e.g. changing the temperature, to the microvalve. The subject devices find use in a variety of applications, including micro-analytical applications.

Chemico-mechanical microvalve and devices comprising the same

Miniaturized planar column devices for use in a liquid phase separation apparatus are described. The devices include microstructures that have been fabricated by laser ablation in a variety of novel support substrates. Devices formed according to the methods of the invention include associated laser-ablated features required for function, such as on-device reservoirs or makeup flow compartments, analyte detection means and sample injection means. The miniaturized columns can be used in an apparatus intended for analysis of either small and/or macromolecular solutes in the liquid phase which employs chromatographic, electrophoretic or electrochromatographic separation means. The apparatus can include a variety of optional injection means, manifolds, keeper means, post column collection means, and combinations thereof.

Miniaturized planar columns for use in a liquid phase separation apparatus

Device for high throughout sample processing, analysis and collection, and methods of use thereof

A miniaturized total analysis system with an in-line NMR detection compartment and an NMR rf microcoil detector is described for use in liquid phase analysis. The device is formed by microfabrication of microstructures in novel support substrates. The NMR detector coil may be fabricated directly in the support body at the point of detection or, alternatively, may be formed as part of a modular structure that is insertable into the device at the point of detection. In addition, an integrated device for sample preparation and NMR detection is provided comprising the miniaturized total analysis system and a miniature magnet configured to accept the miniaturized total analysis system, wherein the device is capable of generating an NMR spectrum. The invention herein is used for the analysis of small and/or macromolecular and/or other solutes in the liquid phase.

Integrated miniaturized device for processing and NMR detection of liquid phase samples

Miniaturized planar column devices are described for use in liquid phase analysis, the devices comprising microstructures fabricated by laser ablation in a variety of novel support substrates. Devices formed according to the invention include associated laser-ablated features required for function, such as analyte detection means and fluid communication means. Miniaturized columns constructed under the invention find use in any analysis system performed on either small and/or macromolecular solutes in the liquid phase and may employ chromatographic, electrophoretic, electrochromatographic separation means, or any combination thereof.

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Palo Alto, CA, United States of America

Sally A Swedberg