Kyowa Hakko Kogyo Co., Ltd.

Kyowa Hakko Food Specialities Co., Ltd.

Sadao Teshiba

Tatsuro Fujio

Shuichi Ishino

Akihiko Maruyama

Satoshi Koizumi

Hideo Mori

Akihiro Iida

Keiichi Yano

Kazuo Yamaguchi

Hideharu Anazawa

Yoshiharu Yokoo

Yoshiyuki Yonetani

Yukio Hashimoto

Makoto Yagasaki

Hiroaki Motoyama

Masaru Hasegawa

Takahiro Hara

Masaki Azuma

Katsura Kitatsuji

Kazuhisa Iwata

Masaru Arimoto

A substantially pure culture of a microorganism having enzyme activities of pyrG and CCT and carrying a recombinant DNA composed of a DNA fragment containing genes encoding pryG and CCT and a vector.

Process for producing cytidine diphosphate choline

A process for producing cytidine diphosphate choline is provided. The process comprises carrying out an enzymatic reaction using microorganisms having the enzyme activities of cytidine-5&prime;-triphosphate synthetase (pyrG), cholinephosphate cytidylyltransferase (CCT) and choline kinase (CKI) and a microorganism capable of producing uridine-5&prime;-triphosphate from orotic acid as the enzyme sources, and orotic acid and choline and/or phosphorylcholine as the substrates; allowing cytidine diphosphate choline to accumulate in the reaction mixture; and recovering cytidine diphosphate choline from said reaction mixture.

The present invention relates to an inosine-guanosine kinase which catalyzes the reaction of forming 5'-inosinic acid (5'-IMP) from inosine and adenosine triphosphate (ATP) or deoxyadenosine triphosphate (dATP) and the reaction of forming 5'-guanylic acid (5'-GMP) from guanosine and ATP or dATP.

Inosine-guanosine kinase

The present invention provides a process for producing riboflavin efficiently at a low cost, wherein riboflavin is formed and accumulated in a medium by culturing a microorganism carrying a recombinant DNA prepared by inserting into a vector DNA a DNA which is derived from a microorganism belonging to the genus Corynebacterium or Brevibacterium and which, upon introduction into a riboflavin-requiring microorganism, confers on the microorganism the ability to complement its riboflavin requirement.

Process for producing riboflavin

Flavine nucleotides, which are useful as ingredients of nutrient compositions, raw materials for various pharmaceutical products, biochemical research reagents and so on, are produced from flavine nucleotide precursors and ATP by utilizing cells or a culture of a microorganism which belongs to the genus Escherichia, Enterobactor or Pseudomonas and harbors a recombinant DNA comprising a vector DNA and a DNA fragment carrying the genetic information relevant to the synthesis of FMN and/or FAD, or treated cells or a treated culture of the microorganism.

Method of producing flavine nucleotides

The present invention provides a DNA coding for uricase from the genus Cellulomonas and a process for producing uricase using a microorganism which carries a recombinant DNA containing the DNA.

DNA coding for uricase and process for producing uricase

Disclosed is a process for producing ascorbic acid-2-phosphate which comprises the steps of reaction, in aqueous medium, of ascorbic acid with a phosphate donor in the presence of cells, treated cells, a culture broth or a treated culture broth, of a microorganism belonging to the genus Escherichia and carrying a recombinant DNA comprising a gene isolated from a microorganism belonging to the species Pseudomonas azotocolligans and coding for ascorbic acid-phosphorylating enzyme until a recoverable amount of ascorbic acid-2-phosphate is accumulated in the aqueous medium, and recovery of ascorbic acid-2-phosphate therefrom.

Process for producing ascorbic acid-2-phosphate

Disclosed is a process for producing amino acids such as L-threohine and L-lysine, which comprises culturing, in a medium containing methanol as a major carbon source, a microorganism which belongs to the genus Methylobacillus and which has an ability to produce the amino acid(s) and resistance to at least one member selected from the group consisting of L-threonine, L-lysine and an amino acid analogue, said microorganism being obtained by mutation of a parent strain which belongs to the genus Methylobacillus and which has an enhanced sensitivity to at least one of an antibiotic and an amino acid analogue; allowing the amino acid(s) to accumulate in the culture; and recovering the amino acid(s) therefrom.

Growing community of inventors

Machida, Japan

Sadao Teshiba