Sysmex Corporation

Sachiyo Tada

Tomokazu Yoshida

Hideki Ishihara

Keiichiro Shohmi

Shigeki Abe

Kazuki Nakabayashi

Harumi Minekawa

Koichi Yamagata

Yasumasa Akai

Yasuyuki Imura

Masatoshi Yamasaki

Masahiro Nishida

It is intended to provide novel primers for nucleic acid amplification to be used in detecting mRNA of a housekeeping gene, more particularly, confirming the amplification of &#x3b2;-actin or GAPDH. More specifically speaking, primers containing oligonucleotides having nucleotide sequences represented by any of SEQ ID NOS: 2 and 4 to 49 (in the case of &#x3b2;-actin) or oligonucleotides having nucleotide sequences represented by any of SEQ ID NOS: 52 to 96 (in the case of GAPDH) can be selected, combined and used.

Primers for nucleic acid amplification in detecting &#x3b2;-actin and test method using these primers

It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:2 to 341 is constructed. Further, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 270- to 930-th regions of the base sequence represented by SEQ ID NO:342 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:343 to 434 is constructed. Furthermore, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 340 to 490-th regions or the 495- to 1050-th regions of the base sequence represented by SEQ ID NO:435 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:436 to 474 is constructed.

Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers

A method for determining the activity of a cell cycle regulatory factor comprising the steps of:

Method for determining activity of cell cycle regulatory factor and method for diagnosing cancer using the same

In detecting a nucleic acid by using the nucleic acid amplification method, it is intended to shorten the time required for the measurement so as to systematically and efficiently examine a gene, etc. To achieve this object, use is made of a method of directly amplifying a nucleic acid by treating a collected biological sample wherein a means of inhibiting the degradation activity of the nucleic acid is introduced during or after the step of homogenizing the biological sample and thus the nucleic acid is directly amplified without isolating/purifying the nucleic acid component from the biological sample. In this method, the means of inhibiting the degradation activity of the nucleic acid is introduced at an acidic pH value (more specifically, from pH 2.5 to pH 5) with the use of a salt interacting with a substance inhibiting the nucleic acid amplification reaction and/or the nucleic acid component so as to directly amplify the nucleic acid without isolating/purifying the nucleic acid component.

Method and system for detection of nucleic acids

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Kobe, Japan

Sachiyo Tada