Director-general of Agency of Industrial Science and Technology

Agency of Industrial Science and Technology Ministry of International

Sachio Miyairi

Koichi Honda

Ikuo Matsui

Kazuhiko Ishikawa

Akira Yabe

Hideaki Tanaka

There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.

Variant-type carbohydrate hydrolase, variant gene of the enzyme and

A given enzyme is immobilized by a method comprising the steps of mixing the enzyme with an azido compound and a high molecular compound as a carrier, forming the resultant mixture in a desired shape, drying the formed mixture and exposing the formed and dried mixture to light for thereby causing the enzyme to be bound to the azido group of the azido compound and to be bound to the high molecular compound.

Enzyme immobilization with azido compounds

Growing community of inventors

Tsukuba, Japan

Sachio Miyairi