National Agriculture and Food Research Organization

Bio-oriented Technology Research Advancement Institution

Director of National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries

National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries

Sachiko Machida

Kiyoshi Hayashi

Takeshi Takaha

Kazutoshi Fujii

Yoshinobu Terada

Hideki Sezutsu

Mayumi Kameyama

Toshiro Kobori

Miyuki Kuramochi

Mari Yamamoto

Ken-ichiro Tatematsu

The present invention pertains to a silkworm-type biotinylated CTLD14 or sRAGE and a method for manufacturing the same. One embodiment of the present invention provides a method for manufacturing biotinylated proteins, wherein the method includes A) a step for inserting a nucleic acid molecule for coding biotin ligase and protein in a coexpressable manner into a silkworm or a living organism that imparts sugar chains that are the same as the sugar chains of the silkworm, B) a step for causing the biotin ligase and protein to be expressed by disposing the silkworm or the living organism that imparts sugar chains that are the same as the sugar chains of the silkworm to conditions with which the nucleic acid molecule will carry out expression, and C) a step for administering biotin to the living organism and obtaining the biotinylated protein.

Biotinylated and oxidized LDL receptor and advanced glycation end product receptor produced using genetically engineered silkworm

The present invention provides an artificial chaperon useful for refolding the proteins having low voluntary folding ability and being difficult or unable to be a native form without a second (or assistant) of a molecular chaperon in a short time, and folding said proteins as an active form. The present invention relates to an artificial chaperon kit characterized in that the kit comprises cyclic saccharide cycloamylose and polyoxyethylenic detergent or cyclic saccharide cycloamylose and ionic detergent. The present invention also relates to a method for diluting the denaturant making the protein a denatured state by adding a specific detergent to a denatured protein, and preventing protein molecules from aggregation, thereafter adding cyclic saccharide cycloamylose, utilizing the inclusion ability thereof to strip detergent, accelerating the proper folding of protein into a correct higher-order structure with activity.

Artificial chaperon kit

A method for activating a denatured protein, includes the steps of: adding a detergent to the denatured protein to allow a protein-detergent complex to be formed; and adding high-molecular weight amylose to the protein-detergent complex so that the high-molecular weight amylose removes the detergent.

Method for activating denatured protein

Growing community of inventors

Tsukuba, Japan

Sachiko Machida