Toyo Ink Mfg. Co., Ltd.

Sachiko Higo

Reiko Tanaka

The present invention provides a method for detecting and/or determining the ATP from microorganism cells in a sample, which comprises the steps of: centrifuging the sample and removing the supernatant, thereby forming a microorganism cell pellet; adding to the microorganism cell pellet a buffer containing a protease-free soluble protein and an ATP hydrolase and incubating the mixture at a pH of 6.0-8.0; extracting ATP from the microorganism cells with an added ATP extraction agent; and detecting and/or determining the ATP released from the microorganism cells by bioluminescence analysis. The present invention also provides a test kit for detecting and/or determining the ATP from microorganism cells, which comprises a reagent containing a buffer capable of pH adjustment to 6.0-8.0, a protease-free soluble protein and an ATP hydrolase, a reagent containing an ATP extraction agent, and a bioluminescence reagent.

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Tokyo, Japan

Sachiko Higo