Kureha Kagaku Kogyo Kabushiki Kaisha

Saburo Ueno

Chikao Yoshikumi

Takayoshi Fujii

Fumio Hirose

Toshihiko Wada

Eiichi Takahashi

Yoshio Omura

Minoru Ohara

Satoru Enomoto

Tetsuya Hotta

Yoshio Ohmura

Shigeto Ohtsuka

Koji Satomi

Akio Sasaki

Disclosed is nitrogen-containing polysaccharide substantially free of units having a molecular weight below about 5,000, said nitrogen-containing polysaccharide having been extracted from Coriolus versicolor (Fr.) Quel., substantially all polymer units having a molecular weight below about 5,000 having been removed therefrom, which exhibits antitumor and immunological activity.

Nitrogen-containing polysaccharide

A method of restoring the immune response of a patient having a lowered immune response level is provided, in which the patient is given an effective amount of certain nitrogen-containing polysaccharides derived from Coriolus versicolor (Fr.) Quel, where the polymer units having a molecular weight of below about 5,000 have been removed therefrom.

Disclosed in this invention are protein-bound polysaccharides having antitumor activities. The polysaccharides have molecular weight within a specified range and present a pattern of color reaction peculiar to the saccharides as well as a pattern of color reaction characteristic of amino acids and peptide bonds. The proton nuclear magnetic resonance spectra of the polysaccharides show peculiar absorption bands. The ratio of the polysaccharide portion to protein portion in the polysaccharides is within a specified range. The protein-bound polysaccharides can be obtained by extraction of the mycelia and/or fruit bodies of fungi belonging to Coriolus of Polyporaceae of Basidiomycetes.

Protein-bound polysaccharides

The fungi belonging to the Coriolus genus is first extracted with water or a dilute alkaline solution and then further extracted stepwise by using alkaline solutions with gradually increased concentrations to obtain a nitrogen-containing polysaccharide having an anti-tumor activity in a high yield.

Method of producing nitrogen-containing polysaccharides

A reciprocating type tenderizer is driven by a rotary crank to enable higher operating speeds and much higher cutting edge velocities. The surface of the conveyor is maintained within 1-2 mm of the bottom dead center position of the cutting edges to provide more complete penetration and a torque limiter is provided for interrupting the conveyor movement during cutting. Novel cutting edge configurations are also provided which are suitable for a wide variety of meats.

Meat tenderizer

The fungi belonging to the Coriolus genus are extracted by using a dilute alkaline solution having a concentration within a certain specified range whereby the low molecular weight substances with molecular weight of less than 5,000 are obtained in there extract solution. These low molecular weight substances are separated from the extract solution to obtain a nitrogen-containing polysaccharide having excellent anti-tumor activity.

Polysaccharides are produced by purifying a liquid extract of a mycelium of a strain of fungus species belonging to the class Basidiomycetes or the filtered broth of a cultured medium in which a mycelium of a selected strain of Basidiomycetes has been incubated; the polysaccharides exhibiting an anticarcinogenic activity in mice.

Growing community of inventors

Tokyo, Japan

Saburo Ueno