Singular Genomics Systems, Inc.

Trilink Biotechnologies, Inc.

Sabrina Shore

Eli N Glezer

Daan Witters

Martin Maria Fabani

Ryan Shultzaberger

Gerald Zon

Alexandre Lebedev

Timothy Looney

Natasha Paul

Jonathan Shum

Andrew King

Provided herein are methods for sequencing both strands of a double stranded nucleic acid fragment that improves fidelity and accuracy of a sequence determination compared to traditional next generation sequencing methods. Compositions and kits for use in the methods are also provided.

Linked paired strand sequencing

Disclosed herein, inter alia, are compositions and methods for amplification of nucleic acid templates, including hybridizing a linear polynucleotide to an immobilized primer on a surface, circularizing the linear polynucleotide to form a circular polynucleotide, and extending the primer with a polymerase.

Nucleic acid circularization and amplification on a surface

Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process.

Chemically modified ligase cofactors, donors and acceptors

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San Diego, CA, United States of America

Sabrina Shore