Evonik Degussa Gmbh

Evonik Operations Gmbh

Evonik Industries Ag

Sabine Hafkemeyer

Thomas Haas

Frank Erhardt

Steffen Schaffer

Oliver Thum

Mirja Wessel

Hans-Georg Joerg Hennemann

Jan Christoph Pfeffer

Markus Poetter

Christian Gehring

Eva Maria Wittmann

Thomas Hueller

Jasmin Corthals

Jasmin Gielen

Thomas Hüller

Katharina Berse

Marion Schiebelhut

Thomas Huller

Markus PÖTTER

The invention relates to a method for oxidizing an alkane, comprising contacting the alkane with a type alkB oxidoreductase and using a type alkB oxidoreductase to prepare a mixture of oxidation products of an alkane, wherein the ratio of carboxylic acid to alcohol in the oxidation products is preferably greater than 1:1.

Biological alkane oxidation

A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of &#x3b2;-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyzes one of the reactions of the &#x3b2;-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.

Process for the improved separation of a hydrophobic organic solution from an aqueous culture medium

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Marl, Germany

Sabine Hafkemeyer