California Institute of Technology

Dow Silicones Corporation

Sabine Brinkmann-Chen

David K Romney

Andrew Richard Buller

Christina E Boville

Remkes A Scheele

Christopher K Prier

Philipp Koch

Javier Murciano Calles

Michael Herger

Paul Van Roye

Frances H Arnold

Dimitris Katsoulis

John Roberts

S B Jennifer Kan

Susanne Baehr

This disclosure provides a method of preparing a silanol-functional organosilicon compound with a cytochrome P450 variant that facilitates the oxidization of a silyl hydride group to a silanol group in the presence of oxygen. The method includes combining the cytochrome P450 variant and an organosilicon compound having at least one silicon-bonded hydrogen atom to give a reaction mixture and exposing the reaction mixture to oxygen to oxidize the organosilicon compound, thereby preparing the silanol-functional organosilicon compound. Cytochrome P450 variants suitable for use in the method are also disclosed, along with methods for engineering and optimizing the same. Nucleic acids encoding the cytochrome P450 variants and compositions, expression vectors, and host cells including the same are also disclosed.

Method of preparing silanols with selective cytochrome P450 variants and related compounds and compositions

The present disclosure provides methods for preparing β-substituted tryptophan compounds. The methods include: combining i) an unsubstituted indole or a substituted indole, ii) a β-substituted serine, and iii) a tryptophan synthase β-subunit (i.e., a TrpB); and maintaining the resulting mixture under conditions sufficient to form the β-substituted tryptophan. The TrpB contains at least one amino acid mutation which promotes formation of an amino-acrylate intermediate. New TrpB variants and new β-substituted tryptophan analogs are also described.

Methods and enzyme catalysts for the synthesis of non-canonical amino acids

This disclosure relates to modified tryptophan synthase and more particularly to modified beta-subunits of tryptophan synthase. The disclosure further relates to cells expressing such modified subunits and methods of producing non-canonical amino acids.

Engineered beta-subunit of tryptophan synthase for production of non-canonical amino acids

The present disclosure provides methods for preparing &#x3b2;-substituted tryptophan compounds. The methods include: combining i) an unsubstituted indole or a substituted indole, ii) a &#x3b2;-substituted serine, and iii) a tryptophan synthase &#x3b2;-subunit (i.e., a TrpB); and maintaining the resulting mixture under conditions sufficient to form the &#x3b2;-substituted tryptophan. The TrpB contains at least one amino acid mutation which promotes formation of an amino-acrylate intermediate. New TrpB variants and new &#x3b2;-substituted tryptophan analogs are also described.

Beta-substituted non-canonical amino acids

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Glendale, CA, United States of America

Sabine Brinkmann-Chen