California Institute of Technology

Gevo, Inc.

The Scripps Research Institute

Sabine Bastian

Frances H Arnold

Peter Meinhold

Chi-Huey Wong

Aristos Aristidou

Jun Urano

Catherine Asleson Dundon

Andrew Hawkins

William H Greenberg

Jared C Lewis

Doug Lies

Thomas Buelter

Reid M Renny Feldman

Justas Jancauskas

Ruth Berry

Yuuichi Mitsuda

Michael M Chen

Clay Bennett

Eva Eckl

Reid M Feldman

The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of theclade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

Modified alcohol dehydrogenases for the production of fuels and chemicals

The disclosure relates to engineered P450 polypeptides and use of such polypeptides in chemoenzymatic methods to construct selectively protected carbohydrates, which are useful as building blocks for preparation of carbohydrate derivatives and oligosaccharides

Methods and compositions for preparation of selectively protected carbohydrates

The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

Engineered microorganisms capable of producing target compounds under anaerobic conditions

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Pasadena, CA, United States of America

Sabine Bastian