Institut Pasteur

Ecole Superieure De Physique Et De Chimie Industrielles De La Ville De Paris (espci)

Sabina Chalabaev

Jean-Marc Ghigo

José Dugay

Sylvie Letoffe

This invention encompasses methods of making 1-propanol. In some embodiments the methods comprise providing a cultured bacterial biofilm; culturing the bacterial biofilm under conditions suitable for production of 1-propanol; and collecting 1-propanol produced by the biofilm culture. In some embodiments the methods comprise providing a bacterial culture comprising bacteria and culture media, wherein the culture media comprises a concentration of threonine higher than that present in LB; maintaining the bacterial culture under conditions suitable for production of 1-propanol; and collecting 1-propanol produced by the culture. This invention also encompasses bacterial culture systems. In some embodiments the bacterial culture systems comprise a bacterial biofilm comprising bacteria growing on an artificial solid substrate; culture media; 1-propanol in liquid and/or gas form; and a collection device configured to collect 1-propanol produced by the culture. In come embodiments the culture systems comprise bacteria; culture media, wherein the culture media comprises a concentration of threonine higher than that present in LB; 1-propanol in liquid and/or gas form; and a collection device configured to collect 1-propanol produced by the culture.

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Paris, France

Sabina Chalabaev