Chonnam National University

Sa-Young Min

Jin-Young Lee

Sung-Hwan You

Geun Joong Kim

Jung-Joon Min

Geun-Joong Kim

Eun-Bin Lee

Won Ji Park

Provided is a ramp tag capable of solving instability in translation rate resulting from poor compatibility between codons in a foreign gene and a host when expressing a recombinant protein in. Unlike the conventional codon optimization or codon deoptimization method for solving the problem of rare codons, the present invention increases an expression efficiency of a target protein by merely having the ramp tag be fused with a target gene or independently expressed, without changing the original codon sequence, thereby allowing tRNA to be reused. Thus, the present invention provides a novel method for increasing recombinant protein expression which is capable of reducing costs and time in comparison to the codon optimization method that artificially synthesizes DNA sequences. Therefore, it is expected that the method of the present invention will be able to be used in production of high value-added pharmaceuticals or industrial enzymes.

Universal protein overexpression tag comprising ramp function, and application thereof

A drug delivery system composition includessp. bacteria orsp. bacteria-derived minicells. The drug delivery system composition is safer for use in human bodies than other bacteria (for example,(),sp. bacteria,sp. bacteria, or the like), or other bacteria-derived drug delivery system. When an anti-cancer drug protein expression construct (protein expression recombinant vector or the like) is included, over-expression of an anti-cancer drug protein, effective protein expression control in vivo, and targeting technique using expression of targeting factor. The drug delivery system composition enables stable drug delivery in vivo, thereby maximizing anti-cancer therapeutic effects.

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Growing community of inventors

Gwangju, South Korea

Sa-Young Min