Genetics Institute, Inc.

S Robert Adamson

Yen-Tung Luan

Denis Drapeau

Randal J Kaufman

Paul Thoday

Douglas A Miller

Cell culture media are provided containing high L-cystine concentration and low L-glutamic acid concentration. The media are useful for recombinant production of proteins using mammalian cell cultures.

Medium and methods for culturing mammalian cho cells

Methods and nutrient media are disclosed useful for adapting mammalian cell lines to culture at increased cell densities.

Adaption of mammalian cell lines to high cell densities

An improved method for producing Factor VIII:c-type proteins is disclosed which involves culturing mammalian cells which are capable of expressing the protein. In accordance with this invention the cells are cultured in a medium containing an effective amount of a substance comprising (a) von Willebrand Factor-type protein, (b) a phospholipid or phospholipid mixture, or a mixture of (a) and (b).

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Chelmsford, MA, United States of America

S Robert Adamson