Scios Nova, Inc.

California Biotechnology Inc.

S Joseph Tarnowski

Stewart A Thompson

Sandra Hilliker

W Scott Willett

Linda C Foster

Kate B Silverness

Trina A Scheuermann

An improved method for expressing peptides as fusion proteins, uses a carrier for a heterologous peptide to provide a fusion protein having a high pI. The high isoelectric point facilitates separation of the fusion protein from all other host cell proteins, and separation of the carrier from the peptide after cleavage.

Expression of recombinant polypeptides with improved purification

Pharmaceutical formulations and a method for their preparation are provided. These formulations provide a stabilized basic fibroblast growth factor (bFGF) which is less susceptible to oxidation or metal-induced aggregation by including an amount of a chelating agent effective to stabilize the bFGF.

Formulations for stabilizing fibroblast growth factor

This invention relates to a method of isolating and purifying proteins such as fibroblast growth factor using metal chelate affinity column chromatography and without the use of heparin. Also disclosed is a method of recovering bFGF multimers and purifying bFGF using metal chelate affinity column chromatography in the absence of heparin.

Method to purify basic fibroblast growth factor

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Sunnyvale, CA, United States of America

S Joseph Tarnowski