Olympus Corporation

Nimura Genetic Solutions Co., Ltd.

Perak State Development Corporation

Ryutaro Akiyoshi

Hirobumi Suzuki

Katsunori Ogo

Takashi Kinebuchi

Mariko Murai

A selection method of an iPS cell includes: at a reprogramming process to culture a cell including a plurality of combinations of initializing factors labelled with luminescent genes that are different with each other, acquiring a photon number per unit area or a photon number per unit time of each of the luminescent genes of the cell; judging whether the acquired photon number is more than a threshold that is predetermined for the acquired photon number; and when the acquired photon number is more than the threshold, selecting this cell as an objective cell for a next process.

Selection method of iPS cell, preparation method of iPS cell, and control device

The present invention relates to a luciferase derived from a Malaysianfirefly, the luciferase having a maximum luminescent wavelength of 557 nm at pH 8, or the luciferase inducing luminescence having 1.5 times or more the luminous intensity of luminescence induced byfirefly luciferase.

Firefly luciferase

An object of the invention is to provide a novel and useful luciferase. The luciferase according to the embodiments of the invention is derived from

Luciferase derived from lucidina accensa

The present invention relates to a luciferase derived from a star-worm belonging to genus, the luciferase inducing luminescence such that a maximum luminous wavelength falls within a range of 557 to 562 nm over the entire pH range of 5.5 to 8.0, or the luciferase inducing luminescence having 1.5 times the luminous intensity of luminescence induced byfirefly luciferase.

Star-worm luciferase

According to one embodiment, the present invention relates to luciferase derived from Malaysianfirefly, the luciferase having a maximum luminescent wavelength of 580 nm at pH 8, or the luciferase indicating 23.3 times or more of luminescent intensity in comparison to that of Rhodamine 6G.

Disclosed is a luminescence measuring method which can produce a luminous intensity depending on the amount of a substance to be measured even when the substance occurs in a biological sample in an amount equal to or more than a given amount, and which can achieve quantitative measurement. The method is characterized by includes preparing a biological sample containing a luminescence-associated protein which is can react with a substance occurring in the biological sample in amount equal to or more than a given amount and which has a Km value equal to or higher than a predetermined value so that the luminous intensity can be quantified depending on the amount of the substance, measuring the luminescence intensity emitted from the biological sample, and outputting a result of the measurement on a regions and/or part of the biological sample.

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Hachioji, Japan

Ryutaro Akiyoshi