Suntory Holdings Limited

Incorporated Adminstrative Agency, National Agriculture and Food Research Organization

Incorporated Administrative Agency National Agriculture and Food Research Organization

Ryutaro Aida

Naonobu Noda

Sanae Sato

Yoshikazu Tanaka

Satoshi Hongo

Akemi Ohmiya

Yukihisa Katsumoto

Masayoshi Nakayama

Mitsuru Douzono

The purpose of the present invention is to provide a breeding method for a plant having a blue flower color with a simpler blue color development controlling technique, without requiring complex mechanisms for blue color development that have been previously presented and techniques reproducing such mechanisms. Delphinidin-based anthocyanins, in which the both 3' and 5&#x2032;-positions of the anthocyanin B-ring have been glycosylated, and flavone glycosides or flavonol glycosides as copigment are made to coexist in the cells of flower organ such as petals.

Plant having blue flower color and breeding method therefor

Provided are transformedplants having blue flower color, their self-fertilized progenies or cross-fertilized progenies thereof, a vegetative propagated plants thereof, and a part, a tissue or a cell of the plant body. Anthocyanin 3',5&#x2032;-O-glucosyltransferase gene (CtA3&#x2032;5&#x2032;GT) derived fromand flavonoid 3&#x2032;,5&#x2032;-hydroxylase gene derived from(CamF3&#x2032;5&#x2032;H) are coexpressed inpetals.

Creation of chrysanthemum with blue flower color

Disclosed are: a method for producing a chrysanthemum plant having delphinidin-containing petals using a transcriptional regulatory region for a chrysanthemum-derived flavanone 3-hydroxylase (F3H) gene; and a chrysanthemum plant, a progeny or a vegetative proliferation product of the plant, or a part or a tissue of the plant, the progeny or the vegetative proliferation product, and particularly a petal or a cut flower of the plant. In the method for producing a chrysanthemum plant having delphinidin-containing petals, a flavonoid 3',5&#x2032;-hydroxylase (F3&#x2032;5&#x2032;H) is caused to be expressed in a chrysanthemum plant using a transcriptional regulatory region for a chrysanthemum-derived flavanone 3-hydroxylase (F3H) gene.

Method for production of chrysanthemum plant having delphinidin-containing petals

Disclosed are a method for controlling flavonoid synthesis in aplant or non-plant by genetic recombination technology using a transcriptional regulatory region useful for altering flower color, a method for modifying anthocyanins, a method for producing aplant or non-plant containing modified anthocyanins in the petals thereof, and aplant or non-plant, progeny thereof, or vegetatively propagated products, part or tissue thereof transformed with the regulatory region. In the method according to the present invention, an expression vector or expression cassette containing a transcriptional regulatory region of perilla anthocyanin 3-acyltransferase gene, such as a nucleic acid containing the nucleotide sequence indicated in SEQ ID NO. 1, or a transcriptional regulatory region of pansy F3'5&#x2032;H gene, such as the nucleotide sequence indicated in SEQ ID NO. 15, is used.

Method for producing chrysanthemum plant having petals containing modified anthocyanin

Growing community of inventors

Tsukuba, Japan

Ryutaro Aida