Mitsubishi Chemical Corporation

Ajinomoto Co., Ltd.

Ryusuke Aoyama

Hiroyuki Kojima

Kenji Yamagishi

Makoto Murase

Jun Nakamura

Keita Fukui

Masaru Utsunomiya

Mika Moriya

Takanao Matsumoto

Chie Koseki

Yasuyo Saito

Kiyohiko Nishi

Shuichi Yunomura

Kiminori Kawakami

Shigeki Nitta

Sanae Sato

Miki Ikuta

Madoka Yonekura

Akiko Sakamoto

Daisuke Kido

Sanae Koike

The invention relates to a method for treating a solution containing a saccharide comprising a heat treatment step of heating the saccharide solution at a temperature of 100&#xb0; C. or higher and 180&#xb0; C. or lower and a reduction step of reacting a reducing agent with the saccharide solution heated in the heat treatment step, a method for producing a treated saccharide solution using the treatment method, a treated saccharide solution obtained through treatment by the treatment method, a method for producing an organic compound comprising an organic-matter production step of obtaining an organic compound by causing a microorganism capable of producing organic matter to act on an organic raw material containing the treated saccharide solution and a method for culturing a microorganism using the treated saccharide solution as a carbon source.

Treatment method of saccharide solution

Provided is a bacterium which is capable of producing an organic acid and is modified so as to have an enhanced 2-oxoglutarate dehydrogenase activity as compared with that of an unmodified strain. An organic acid such as succinic acid can be produced by culturing the bacterium.

Bacterium having enhanced 2-oxoglutarate dehydrogenase activity

Succinic acid is produced by allowing a bacterium modified to enhance fumarate reductase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. More preferably, succinic acid is produced by allowing a bacterium modified to enhance activities of fumarate reductase and pyruvate carboxylase and decrease lactate dehydrogenase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. Succinic acid is obtained by collecting the produced succinic acid.

Method of producing succinic acid with bacterium comprising a modified fumarate reductase gene or a modified succinate dehydrogenase gene

Non-amino organic acids such as succinic acid, malic acid and fumaric acid are produced by reacting bacterial cells or treated bacterial cells of a coryneform bacterium with an organic raw material in an aqueous medium containing magnesium carbonate and/or magnesium hydroxide, and a certain range of concentration of a monovalent cation, while maintaining the pH within a certain range without increasing the volume of the aqueous medium.

Method for producing non-amino organic acid

Growing community of inventors

Yokohama, Japan

Ryusuke Aoyama