Hiroshima University

Repertoire Genesis Incorporation

Hyogo College of Medicine

Reperptoire Genesis Incorpration

Ryuji Suzuki

Takashi Yamamoto

Tetsushi Sakuma

Tatsuo Ichinohe

Takahiko Miyama

Takakazu Kawase

Yasuko Honjo

Seiji Matsumoto

T cell receptor (TCR) diversity of a subject, including TCR diversity in CD8+ T cell subsets, is used as a predictive indicator of responsiveness of the subject to cancer immunotherapy prior to initiation of the immunotherapy. Exemplified immunotherapy comprises administering an immune checkpoint inhibitor to a subject, wherein a TCR diversity value in CD8+ T cell subsets from the subject, such as CD8+ T cell subpopulations defined by differential cell surface marker expression, is higher than a reference value.

Biomarkers for cancer immunotherapy

The present disclosure provides a technique whereby the influence of an endogenous TCR is eliminated in TCR gene transfer. A TCR gene is edited using a genome editing enzyme, said genome editing enzyme having one characteristic that amino acids at two specific positions in DNA-binding modules contained in a DNA-binding domain thereof show repeating patterns which differ from one module to another among the four DNA-binding modules. Thus, a lowering in the expression efficiency of the transferred TCR caused by mispairing with an endogenous TCR and the occurrence of a self-reactive TCR are avoided.

Full replacement technique for T cell receptor using platinum TALEN

The present disclosure provides a technique for producing regulatory T cells specific to a desired antigen. Regulatory T cells specific to an antigen are produced by a method that includes: a step for identifying a T cell receptor (TCR) clone present in an effector T cell population specific to an antigen in an effector T cell donor; and a step for introducing all or part of the nucleic acid sequence of the TCR&#x3b1; gene and all or part of the nucleic acid sequence of the TCR&#x3b2; gene included in the clone into a regulatory T cell, said step comprising introducing the TCR&#x3b1; and the TCR&#x3b2; so as to be expressed in pairs.

Technique for creating antigen-specific regulatory T cells (Treg) in which effector T cell (Teff) antigen receptors are used

Growing community of inventors

Ibaraki, Japan

Ryuji Suzuki