Agency of Industrial Science and Technology

Other

National Institute of Advanced Industrial Science and Technology

Kankyo Engineering Co., Ltd.

Japan Bioindustry Association

Astellas Pharma Gmbh

Ministry of International Trade & Industry

Agency of Industrial Science and Technology Ministry of International

Japan As Represented by Secretary of Agency of Industrial Science and Technology

Hakuto Co., Ltd.

Kyowa Hokkoh Kogyo Co., Ltd.

Ryuichiro Kurane

Shinya Kurata

Takahiro Kanagawa

Yoichi Kamagata

Kazutaka Yamada

Toyokazu Yokomaku

Osamu Koyama

Kenta Furusho

Tomoo Suzuki

Yoshimasa Takahara

Masaki Torimura

Yasuhiro Nohata

Masakazu Yamaoka

Takayasu Tsuchida

Koji Nagai

Masato Watanabe

Hideki Murata

Ken-ichi Suzuki

Shuichi Ishino

Nakako Arao

Michio Shiomi

Kazuma Kamigiri

Yasuhiro Kawano

Kenichi Suzumura

Toshihiro Yokochi

Tomoo Kazuo

Akihiko Maruyama

Hisaaki Yagi

Toro Nakahara

Akira Manome

Takuya Kitamura

Seiji Sugimoto

Satoru Kurozumi

Akira Yotsuji

A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.

Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method

A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises:

Methods for monitoring PCR amplification with fluorescently labeled probes or primers for which fluorescence changes upon hybridization to a target

Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.

Nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method

A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.

Nucleic acid probe and novel method of assaying nucleic acid using the same

This invention relates to drainage process whereinAIK bacterial strain (FERM BP-7308) contacts disposed drainage and the new microorganism can aerobically decompose liquid and solid oil and fat and other organic matter in wide range of temperatures and a drainage process that uses the new microorganism.

Microorganism and drainage method

A microorganism belonging to the genusis cultured and thiopeptide compounds QN3323 and thiocillin I/II compounds are collected from the culture medium. The compounds are useful as drugs to treat infections caused by multidrug-resistant bacteria.

Thiopeptide compounds

Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method

A method for separating genes from viruses existing in hydrosphere in an intact state, a method for fractionating thus separated genes, and a method for analyzing thus fractionated genes.

Methods for separating,fractionating and analyzing gene of virus existing in hydrosphere

Present invention provides a method to efficiently manufacture an oil-containing product including long chain polyunsaturated fatty acid, or a high added value oil containing long chain polyunsaturated fatty acids. The method comprises culturing a microorganism belonging to the Genus Labyrinthula. The Labyrinthula microorganism is cultured in a medium containing oil or fatty acid as a carbon source, and the produced long chain polyunsaturated fatty acid is recovered from the culture. With the present invention, it is possible to efficiently produce from the vegetable oil and the like, an oil containing product comprising long chain polyunsaturated fatty acids such as docosahexanoic acid having a carbon number of 20 or more, and two or more unsaturated bonds, or an oil containing the long chain polyunsaturated fatty acid. Further, the method of the present invention may be used to reform oil by converting the plant oil and like to high added value oil containing the long chain polyunsaturated fatty acid.

Method of producing a polyunsaturated fatty acid containing culture and polyunsaturated fatty acid containing oil using microorganisms

The present invention provides a method for isolating a microbe whereby a sample of microbe cells is encapsulated in agarose gel particulates, wherein some of the particulates contain a single cell, and the other particulates contain more than one cell; incubating the particulates in nutritional and environmental conditions that enable the microbe contained in the sample solution that can grow on a plate of a plate culture method to grow in the agarose gel particulate; and isolating the particulates having single cells from the group of the particulates having more than one cell.

Method for isolating a microbe

Polysaccharide and a method of producing it

A polysaccharide (Biopolymer B-16) being produced by cultivating Alcaligenes latus strain B-16 (FERM-2015) and having at least one function selected from water absorption, moisture absorption, humectant capability, thickening capability, suspension stability, emulsion stability and dispersant capability along with high biodegradability and which can be used without creating any environmental hazard such as secondary pollution, said Biopolymer B-16 consisting essentially of rhamnose, fucose, glucose, mannose and glucuronic acid which are present in a molar ratio of

Polysaccharide, and water absorbent, moisture absorbent or humectant and

Growing community of inventors

Tukuba, Japan

Ryuichiro Kurane