Kaneka Corporation

Ryozo Imai

Naoaki Taoka

Yozo Nagira

Ryuji Miki

Haruyasu Hamada

Keiji Matsumoto

Masahiro Nogawa

Mineo Kojima

Kentaro Sasaki

Myung Hee Kim

A method for editing a plant genome includes coating a microparticle with at least one type of nucleic acid and/or at least one type of protein, introducing a deletion, insertion, or substitution into a target site in the genome of a plant by bombarding a shoot apex of the plant with the coated microparticle using a gene gun, growing the shoot apex bombarded with the coated microparticle to obtain a plant body, and selecting a genome-edited plant body from the plant body. The shoot apex of the plant is selected from the group consisting of a shoot apex of an embryo of a fully mature seed, a shoot apex of a young bud of a tuber, and a shoot apex of a terminal bud or a lateral bud.

Plant genome editing method

A method to transforming a plant includes coating a microparticle having a diameter of 0.3 to 0.9 &#x3bc;m with at least one type of nucleic acid, bombarding a shoot apex of the plant with the coated microparticle using a gene gun, growing the shoot apex bombarded with the coated microparticle to obtain a plant body, and selecting a transformed plant body from the plant body. The shoot apex is selected from the group consisting of a shoot apex of an embryo of a fully mature seed, a shoot apex of a young bud of a tuber, and a shoot apex of a terminal bud or a lateral bud.

Method for creating transformed plant

This invention provides a method of plant transformation viacomprising inoculating and infecting a plant with ancarrying a foreign-gene-containing vector under application of a plant growth-inhibiting hormone. This invention also provides a method of plant transformation viacomprising transforming a plant with ancarrying a vector comprising a T-DNA region in which a foreign gene is contained but no selectable marker gene is contained.

Plant transformation method using plant growth inhibiting hormone

Growing community of inventors

Hokkaido, Japan

Ryozo Imai