Japan Science and Technology Agency

The University of Tokyo

Osaka University

Fukoku Co., Ltd.

Ryota Iino

Hiroyuki Noji

Suguru Araki

Toshiharu Suzuki

Jun Ohta

Takashi Tokuda

Megumi Shio

Soo Hyeon Kim

Toshihiko Noda

Yoshimi Matsumoto

Masasuke Yoshida

Kiyotaka Sasagawa

Kohei Hayama

Shouichi Sakakihara

Kunihiko Nishino

Akihito Yamaguchi

This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent () containing beads (),(') into a space () between (a) a lower layer section () including a plurality of receptacles () each of which is capable of storing only one of the beads (),(&#x2032;) and which are separated from each other by a side wall () having a hydrophobic upper surface and (b) an upper layer section () facing a surface of the lower layer section () on which surface the plurality of receptacles () are provided; and (ii) a step of introducing a hydrophobic solvent () into the space (), the step (ii) being carried out after the step (i).

Method for detecting target molecule

This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent () containing beads (),() into a space () between (a) a lower layer section () including a plurality of receptacles () each of which is capable of storing only one of the beads (),() and which are separated from each other by a side wall () having a hydrophobic upper surface and (b) an upper layer section () facing a surface of the lower layer section () on which surface the plurality of receptacles () are provided; and (ii) a step of introducing a hydrophobic solvent () into the space (), the step (ii) being carried out after the step (i).

Kit for sealing beads and detecting target molecule

There are provided a detector and a detection method capable of detecting a biological sample with high sensitivity. A detector is provided with a microchamber array including a plurality of storage sections to be filled with a hydrophilic solvent containing a biological sample; and an image sensor in which picture elements are disposed corresponding to the storage sections, wherein the microchamber array includes a flow channel in communication with openings of the storage sections; a hydrophobic solvent supply unit arranged in continuity with the flow channel; and a through-hole which allows the hydrophilic solvent to enter and exit the flow channel, and the hydrophobic solvent supply unit flows a hydrophobic solvent into the flow channel by an externally-applied force.

Detector and detection method

The present disclosure describes a method capable of easily and rapidly inspecting the susceptibility of bacteria or fungi to an antimicrobial drug. The inspection method of the present disclosure is a method for inspecting susceptibility of bacteria or fungi to an antimicrobial drug using a micro-device having flow channels, including: incubating a mixture of the antimicrobial drug and a suspension to be inspected in the flow channels of the micro-device; and detecting bacteria or fungi derived from the suspension to be inspected in an observation area of the flow channels of the micro-device. The detecting step can be performed by detecting an increase or decrease in the number of or a change in shape of bacteria or fungi derived from the suspension to be inspected in the observation area by a microscope or the like.

Method for inspecting susceptibility of bacteria or fungi to antimicrobial drug and system for use in the same

Bead trapping method and method for detecting target molecule

An illumination system uses rotatable, polarized illumination optics to detect the direction of highly efficient excitation of fluorescent dyes coupled to a sample, or the absorption transition moment, using information on the direction of maximal fluorescence intensity, in which it is possible to detect individual dynamic changes in the internal structure or orientation of an entire protein molecule by coupling a single fluorescent dye molecule to the protein molecule. The polarized total internal reflection illumination optical system by rotary annulus light is also an illumination optical system in which laser beams are introduced into the objective lens of a microscope at the peripheral region by means of the rotatable illumination direction of the laser beams, and is designed to illuminate by the evanescent field that contains only transverse components that are perpendicular to the direction of radiation from the optical axis of the objective lens.

Polarized total internal reflection illumination optical system by rotary annulus light

Growing community of inventors

Tokyo, Japan

Ryota Iino