Tokyo Metropolitan Institute of Medical Science

Toray Industries, Inc.

Japan As Represented by Director-general of National Institute of Infectious Diseases

Ryosuke Suzuki

Tetsuro Suzuki

Takaji Wakita

Koji Ishii

Tatsuo Miyamura

Saburo Sone

Jun-ichi Tanabe

The present invention relates to a method for producing a recombinant hepatitis C virus-like particle comprising the steps of introducing into (i) a cell in which an RNA replicon comprising a nucleotide sequence comprising the 5' untranslated region, the nucleotide sequence coding for the NS3, NS4A, NS4B, NS5A, and NS5B proteins, and the 3&#x2032; untranslated region of a genome RNA derived from a hepatitis C virus strain autonomously replicates, (ii) a vector expressing the Core, E1, E2, and p7 proteins derived from a hepatitis C virus strain that is the same as or different from that as defined in the above (i), culturing the cell, and recovering the produced virus-like particle, and a recombinant hepatitis C virus particle produced by this method.

Recombinant human hepatitis C virus-like particle and method for producing the same

The present invention relates to a method for producing infectious hepatitis C virus (HCV) particles, comprising a step of introducing an expression vector into a cell that allows HCV proliferation, such expression vector comprising: DNA sequences encoding the 5' untranslated region, structural proteins, and, if necessary, non-structural proteins of HCV and DNA sequences encoding non-structural proteins and the 3&#x2032; untranslated region derived from the HCV JFH1 strain, which are located downstream of a polymerase I promoter; and a DNA fragment containing an RNA polymerase I terminator, which is located further downstream thereof.

High production system for infectious hepatitis C virus particle

Growing community of inventors

Chiba, Japan

Ryosuke Suzuki